Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Dec 1;232(2):425–430. doi: 10.1042/bj2320425

Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin.

M T Téllez-Iñón, R M Ulloa, G C Glikin, H N Torres
PMCID: PMC1152896  PMID: 3004404

Abstract

Activation of cyclic AMP phosphodiesterase I by brain or Neurospora calmodulin was studied. The stimulation required micromolar concentrations of Ca2+, and it was observed at cyclic AMP concentrations between 0.1 and 500 microM. Activation was blocked by EDTA and some neuroleptic drugs such as chlorpromazine and fluphenazine. These drugs inhibit the elongation of N. crassa wild-type aerial hyphae. These results reinforce the evidence towards the recognition of Ca2+-calmodulin as one of the systems controlling cyclic nucleotide concentrations in Neurospora.

Full text

PDF
423

Images in this article

427Image
on p.427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartfai T. Preparation of metal-chelate complexes and the design of steady-state kinetic experiments involving metal nucleotide complexes. Adv Cyclic Nucleotide Res. 1979;10:219–242. [PubMed] [Google Scholar]
  2. Bazari W. L., Clarke M. Characterization of a novel calmodulin from Dictyostelium discoideum. J Biol Chem. 1981 Apr 10;256(7):3598–3603. [PubMed] [Google Scholar]
  3. Cheung W. Y. Calmodulin plays a pivotal role in cellular regulation. Science. 1980 Jan 4;207(4426):19–27. doi: 10.1126/science.6243188. [DOI] [PubMed] [Google Scholar]
  4. Clarke M., Bazari W. L., Kayman S. C. Isolation and properties of calmodulin from Dictyostelium discoideum. J Bacteriol. 1980 Jan;141(1):397–400. doi: 10.1128/jb.141.1.397-400.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Flawiá M. M., Terenzi H. F., Torres H. N. Characterization of Neurospora crassa mutant stratins deficient in adenylate cyclase activity. Arch Biochem Biophys. 1977 Apr 30;180(2):334–342. doi: 10.1016/0003-9861(77)90046-7. [DOI] [PubMed] [Google Scholar]
  6. Jamieson G. A., Jr, Vanaman T. C. Calcium-dependent affinity chromatography of calmodulin on an immobilized phenothiazine. Biochem Biophys Res Commun. 1979 Oct 12;90(3):1048–1056. doi: 10.1016/0006-291x(79)91932-6. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Londesborough J. Quantitative estimation of 3'5' cyclic AMP phosphodiesterase using anion exchange resin in a batch process. Anal Biochem. 1976 Apr;71(2):623–628. doi: 10.1016/s0003-2697(76)80037-1. [DOI] [PubMed] [Google Scholar]
  9. Ortega Perez R., Van Tuinen D., Marmé D., Cox J. A., Turian G. Purification and identification of calmodulin from Neurospora crassa. FEBS Lett. 1981 Oct 26;133(2):205–208. doi: 10.1016/0014-5793(81)80506-6. [DOI] [PubMed] [Google Scholar]
  10. Ortega Perez R., Van Tuinen D., Marmé D., Turian G. Calmodulin-stimulated cyclic nucleotide phosphodiesterase from Neurospora crassa. Biochim Biophys Acta. 1983 Jul 5;758(1):84–87. doi: 10.1016/0304-4165(83)90012-0. [DOI] [PubMed] [Google Scholar]
  11. Perkins D D. New Markers and Multiple Point Linkage Data in Neurospora. Genetics. 1959 Nov;44(6):1185–1208. doi: 10.1093/genetics/44.6.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reig J. A., Kornblihtt A. R., Flawiá M. M., Torres H. N. Soluble adenylate cyclase activity in Neurospora crassa. Biochem J. 1982 Oct 1;207(1):43–49. doi: 10.1042/bj2070043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reig J. A., Téllez-Iñn M. T., Flawiá M. M., Torres H. N. Activation of Neurospora crassa soluble adenylate cyclase by calmodulin. Biochem J. 1984 Jul 15;221(2):541–543. doi: 10.1042/bj2210541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reissig J. L., Kinney S. G. Calcium as a branching signal in Neurospora crassa. J Bacteriol. 1983 Jun;154(3):1397–1402. doi: 10.1128/jb.154.3.1397-1402.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Terenzi H. F., Flawia M. M., Tellez-Inon M. T., Torres H. N. Control of Neurospora crassa morphology by cyclic adenosine 3', 5'-monophosphate and dibutyryl cyclic adenosine 3', 5'-monophosphate. J Bacteriol. 1976 Apr;126(1):91–99. doi: 10.1128/jb.126.1.91-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Terenzi H. F., Flawiá M. M., Torres H. N. A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity. Biochem Biophys Res Commun. 1974 Jun 18;58(4):990–996. doi: 10.1016/s0006-291x(74)80241-x. [DOI] [PubMed] [Google Scholar]
  17. Thompson W. J., Appleman M. M. Multiple cyclic nucleotide phosphodiesterase activities from rat brain. Biochemistry. 1971 Jan 19;10(2):311–316. [PubMed] [Google Scholar]
  18. Trevillyan J. M., Pall M. L. Control of cyclic adenosine 3',5'-monophosphate levels by depolarizing agents in fungi. J Bacteriol. 1979 May;138(2):397–403. doi: 10.1128/jb.138.2.397-403.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Téllez-Iñn M. T., Glikin G. C., Torres H. N. Cyclic nucleotide phosphodiesterase activities in Neurospora crassa. Biochem J. 1982 Jun 1;203(3):611–616. doi: 10.1042/bj2030611. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES