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. 1985 Dec 1;232(2):577–583. doi: 10.1042/bj2320577

Novel retinoid-binding proteins from filarial parasites.

B P Sani, A Vaid, J C Comley, J A Montgomery
PMCID: PMC1152918  PMID: 3004410

Abstract

The present study deals with the discovery and partial characterization of specific binding proteins for retinol and retinoic acid from filarial parasites (worms of the superfamily Filarioidea), including those from two species of Onchocerca. These binding proteins, which are distinct in their physicochemical properties and in the mode of ligand interactions from the host-tissue retinoid-binding proteins, may be involved in the mediation of the putative biological roles of retinoids in the control of parasitic growth, differentiation and reproduction. Parasite retinol-binding protein and retinoic acid-binding protein exhibited specificity for binding retinol and retinoic acid respectively. Both the binding proteins showed an s20,w value of 2.0 S. On gel filtration, both proteins were retarded to a position corresponding to the same molecular size (19.0 kDa). On preparative columns, the parasite binding proteins exhibited isoelectric points at pH 5.7 and 5.75. Unlike the retinoid-binding proteins of mammalian and avian origin, the parasite retinoid-binding proteins showed a lack of mercurial sensitivity in ligand binding. The comparative amounts of retinoic acid-binding protein in five parasites, Onchocerca volvulus, Onchocerca gibsoni, Dipetalonema viteae, Brugia pahangi and Dirofilaria immitis, were between 2.7 and 3.1 pmol of retinoic acid bound/mg of extractable protein. However, the levels of parasite retinol-binding protein were between 4.8 and 5.8 pmol/mg, which is considerably higher than the corresponding levels of cellular retinol-binding protein of mammalian and avian origin. Both retinol- and retinoic acid-binding-protein levels in O. volvulus-infected human nodules and O. gibsoni-infected bovine nodules were similar to their levels in mammalian tissues. Also, these nodular binding proteins, like the host-binding proteins, exhibited mercurial sensitivity to ligand interactions.

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Selected References

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  1. Bashor M. M., Toft D. O., Chytil F. In vitro binding of retinol to rat-tissue components. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3483–3487. doi: 10.1073/pnas.70.12.3483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bollag W. Prophylaxis of chemically induced benign and malignant epithelial tumors by vitamin A acid (retinoic acid). Eur J Cancer. 1972 Dec;8(6):689–693. doi: 10.1016/0014-2964(72)90153-3. [DOI] [PubMed] [Google Scholar]
  3. Comley J. C., Jaffe J. J. The conversion of exogenous retinol and related compounds into retinyl phosphate mannose by adult Brugia pahangi in vitro. Biochem J. 1983 Aug 15;214(2):367–376. doi: 10.1042/bj2140367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dowling J. E., Wald G. THE BIOLOGICAL FUNCTION OF VITAMIN A ACID. Proc Natl Acad Sci U S A. 1960 May;46(5):587–608. doi: 10.1073/pnas.46.5.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goodman D. S. Retinoid-binding proteins. J Am Acad Dermatol. 1982 Apr;6(4 Pt 2 Suppl):583–590. doi: 10.1016/s0190-9622(82)70065-9. [DOI] [PubMed] [Google Scholar]
  6. Jetten A. M., Jetten M. E. Possible role of retinoic acid binding protein in retinoid stimulation of embryonal carcinoma cell differentiation. Nature. 1979 Mar 8;278(5700):180–182. doi: 10.1038/278180a0. [DOI] [PubMed] [Google Scholar]
  7. Juhász S., Babos S. Studies of parasite metabolism. I. In vitro examination of the vitamin A metabolism of Ascaris suum. Acta Vet Acad Sci Hung. 1969;19(3):239–251. [PubMed] [Google Scholar]
  8. Liau G., Ong D. E., Chytil F. Interaction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components. J Cell Biol. 1981 Oct;91(1):63–68. doi: 10.1083/jcb.91.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mahalanabis D., Jalan K. N., Maitra T. K., Agarwal S. K. Vitamin A absorption in ascariasis. Am J Clin Nutr. 1976 Dec;29(12):1372–1375. doi: 10.1093/ajcn/29.12.1372. [DOI] [PubMed] [Google Scholar]
  10. Ong D. E., Chytil F. Cellular retinoic acid-binding protein from rat testis. Purification and characterization. J Biol Chem. 1978 Jul 10;253(13):4551–4554. [PubMed] [Google Scholar]
  11. Ong D. E., Chytil F. Specificity of cellular retinol-binding protein for compounds with vitamin A activity. Nature. 1975 May 1;255(5503):74–75. doi: 10.1038/255074a0. [DOI] [PubMed] [Google Scholar]
  12. RODGER F. C. A review of recent advances in scientific knowledge of the symptomatology, pathology and pathogenesis of onchoceral infections. Bull World Health Organ. 1962;27:429–448. [PMC free article] [PubMed] [Google Scholar]
  13. Ross A. C., Takahashi Y. I., Goodman D. S. The binding protein for retinol from rat testis cytosol. Isolation and partial characterization. J Biol Chem. 1978 Sep 25;253(18):6591–6598. [PubMed] [Google Scholar]
  14. Saffiotti U., Montesano R., Sellakumar A. R., Borg S. A. Experimental cancer of the lung. Inhibition by vitamin A of the induction of tracheobronchial squamous metaplasia and squamous cell tumors. Cancer. 1967 May;20(5):857–864. doi: 10.1002/1097-0142(1967)20:5<857::aid-cncr2820200545>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  15. Sani B. P., Banerjee C. K., Peckham J. C. The presence of binding proteins for retinoic acid and dihydrotestosterone in murine and human colon tumors. Cancer. 1980 Dec 1;46(11):2421–2429. doi: 10.1002/1097-0142(19801201)46:11<2421::aid-cncr2820461121>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  16. Sani B. P., Banerjee C. K. Purification and properties of retinoic acid-binding protein from chick-embryo skin. Biochem J. 1978 Aug 1;173(2):643–649. doi: 10.1042/bj1730643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sani B. P., Comley J. C. Role of retinoids and their binding proteins in filarial parasites and host tissues. Trop Med Parasitol. 1985;36 (Suppl 1):20–23. [PubMed] [Google Scholar]
  18. Sani B. P., Condon S. M., Banerjee C. K. Purification and properties of retinol- and retinoic acid-binding proteins from a transplantable mouse colon tumor. Biochim Biophys Acta. 1980 Jul 24;624(1):226–236. doi: 10.1016/0005-2795(80)90242-1. [DOI] [PubMed] [Google Scholar]
  19. Sani B. P., Donovan M. K. Localization of retinoic acid-binding protein in nuclei and the nuclear uptake of retinoic acid. Cancer Res. 1979 Jul;39(7 Pt 1):2492–2496. [PubMed] [Google Scholar]
  20. Sani B. P., Hill D. L. A retinoic acid-binding protein from chick embryo skin. Cancer Res. 1976 Feb;36(2 Pt 1):409–413. [PubMed] [Google Scholar]
  21. Sani B. P., Hill D. L. Retinoic acid: a binding protein in chick embryo metatarsal skin. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1276–1282. doi: 10.1016/s0006-291x(74)80422-5. [DOI] [PubMed] [Google Scholar]
  22. Sani B. P. Localization of retinoic acid-binding protein in nuclei. Biochem Biophys Res Commun. 1977 Mar 7;75(1):7–12. doi: 10.1016/0006-291x(77)91281-5. [DOI] [PubMed] [Google Scholar]
  23. Sani B. P., Titus B. C., Banerjee C. K. Determination of binding affinities of retinoids to retinoic acid-binding protein and serum albumin. Biochem J. 1978 Jun 1;171(3):711–717. doi: 10.1042/bj1710711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sanl B. P., Corbett T. H. Retinoic acid-binding protein in normal tissues and experimental tumors. Cancer Res. 1977 Jan;37(1):209–213. [PubMed] [Google Scholar]
  25. Storey D. M. Vitamin A deficiency and the development of Litomosoides carinii (Nematoda, Filarioidea) in cotton rats. Z Parasitenkd. 1982;67(3):309–315. doi: 10.1007/BF00927666. [DOI] [PubMed] [Google Scholar]
  26. Sturchler D., Wyss F., Hanck A. Retinol, onchocerciasis and Onchocerca volvulus. Trans R Soc Trop Med Hyg. 1981;75(4):617–617. doi: 10.1016/0035-9203(81)90230-3. [DOI] [PubMed] [Google Scholar]
  27. Stürchler D., Holzer B., Hanck A., Degrémont A. The influence of schistosomiasis on the serum concentrations of retinol and retinol binding protein of a rural population in Liberia. Acta Trop. 1983 Sep;40(3):261–269. [PubMed] [Google Scholar]
  28. Turchetti-Maia R. M., Bambirra E. A., Coelho P. M., Moraes e Santos T., Vieira E. C. The association of Schistosoma mansoni infection with deficiency of vitamin A in mice. Rev Inst Med Trop Sao Paulo. 1983 Sep-Oct;25(5):215–222. [PubMed] [Google Scholar]
  29. Wiggert B., Russell P., Lewis M., Chader G. Differential binding to soluble nuclear receptors and effects on cell viability of retinol and retinoic acid in cultured retinoblastoma cells. Biochem Biophys Res Commun. 1977 Nov 7;79(1):218–225. doi: 10.1016/0006-291x(77)90083-3. [DOI] [PubMed] [Google Scholar]

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