Fig. 5.

A population of undifferentiated cells persists through the accelerated larval period of He. (A) UMAP of He development highlighting the undifferentiated cell cluster (pink), with an inset of the undifferentiated cell region of the UMAP colored by time point. (B) Plot showing the number of cells belonging to the undifferentiated cell cluster at each time point in the scRNA-seq time course. (C) Transition table showing the Waddington-OT trajectory inference results for the undifferentiated cell cluster. Transition probability values indicate the likelihood that cells beginning in the undifferentiated cell cluster transition into a different cell type identity. (D) Chart showing the top enriched GO terms in the undifferentiated cluster, colored by adjusted P-value. (E) UMAP of He development showing co-expression between nanos, seawi and vasa, three of the main markers of the germline in planktotrophic sea urchins.