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. 2005 Apr;25(2):393–406. doi: 10.1007/s10571-005-3066-7

Electrophysiological Responses of Nucleus Tractus Solitarius Neurons to CCK and Gastric Distension in Newborn Lambs

Rosalinda Guevara-Guzmán 1,4,✉, Frederic Lévy 2, Andre Jean 3, Raymond Nowak 2
PMCID: PMC11529607  PMID: 16047548

Abstract

The effect of cervical vagus nerve stimulation, gastric distension and CCK-8S administration was studied on the activity of 120 neurons located in the nucleus tractus solitarius (NTS) of anesthetized newborn lambs. One hundred cells responded to the three different inputs.

The distribution of the cells in the NTS was from 3 mm rostral to 3 mm caudal to the obex, the major responsive cells being located at the level of the obex. Neurons were either excited or inhibited by gastric distension and CCK-8S, and the responses to these two stimuli were always in the same direction. A small number of cells responded to gastric distension and CCK-8S but not to vagus nerve stimulation.

Injection of the CCK-A receptor antagonist 2-NAP abolished both the responses to CCK-8S and to gastric distension. The results are consistent with the idea that CCK-8S acts directly on vagal mechanoreceptive endings in the gastric corpus close to duodenum.

These results from lambs may reflect the pathway by which gastric distension and peripheral CCK-8S modulate NTS cells activity during colostrum ingestion, which could in turn activate structures related to learning and memory processes involved in the development of mother preference.

Keywords: nucleus tractus solitarius, gastric distension, vagus nerve, cholecystokinin, CCK-A receptor, 2-NAP, lamb, newborn

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