Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1985 Dec 15;232(3):859–862. doi: 10.1042/bj2320859

Structural differences between plasma-membrane 5'-nucleotidase in different cell types as evidenced by antibodies.

J Harb, K Meflah, S Bernard
PMCID: PMC1152961  PMID: 2418821

Abstract

Antibodies raised against bovine 5'-nucleotidase inhibit this enzyme as well as 5'-nucleotidase from other bovine tissues, showing common structure(s) between these proteins. However, an IgG fraction directed against the glucidic moiety of the liver enzyme did not cross-react with the enzyme from lymphocyte or caudate nuclei, a clear indication that within the same species the 5'-nucleotidase differs from one cell type to another. In addition, immunoblots after electrophoresis show that the previous antibodies recognize 5'-nucleotidase from human, mouse or chicken origin. However, only human 5'-nucleotidase activity can be inhibited by the antibodies. Thus at least three groups of antigenic determinants must exist on the 5'-nucleotidase: one related to the glucidic moiety of the glycoprotein whose binding inhibits the enzyme activity, another related to the catalytic site, as its binding also led to enzyme inhibition, and a last one of structural nature. It seems that the third group of determinant is common to many species, whereas the second one is more restricted.

Full text

PDF
858

Images in this article

860Image
on p.860

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alhadeff J. A., Andrews-Smith G. L. Radioimmunochemical evidence for a role of carbohydrate in antigenic determinant(s) on human liver alpha-L-fucosidase. Biochem J. 1984 Oct 15;223(2):293–298. doi: 10.1042/bj2230293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailyes E. M., Soos M., Jackson P., Newby A. C., Siddle K., Luzio J. P. The existence and properties of two dimers of rat liver ecto-5'-nucleotidase. Biochem J. 1984 Jul 15;221(2):369–377. doi: 10.1042/bj2210369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carson D. A., Seegmiller J. E. Effect of adenosine deaminase inhibition upon human lymphocyte blastogenesis. J Clin Invest. 1976 Feb;57(2):274–282. doi: 10.1172/JCI108278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DePierre J. W., Karnovsky M. L. Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I. Evidence for an ecto-adenosine monophosphatase, adenosine triphosphatase, and -p-nitrophenyl phosphates. J Biol Chem. 1974 Nov 25;249(22):7111–7120. [PubMed] [Google Scholar]
  5. EMMELOT P., BOS C. J., BENEDETTI E. L., RUEMKE P. STUDIES ON PLASMA MEMBRANES. I. CHEMICAL COMPOSITION AND ENZYME CONTENT OF PLASMA MEMBRANES ISOLATED FROM RAT LIVER. Biochim Biophys Acta. 1964 Jul 15;90:126–145. doi: 10.1016/0304-4165(64)90125-4. [DOI] [PubMed] [Google Scholar]
  6. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  7. Ferber E., Resch K., Wallach D. F., Imm W. Isolation and characterization of lymphocyte plasma membranes. Biochim Biophys Acta. 1972 May 9;266(2):494–504. doi: 10.1016/0005-2736(72)90105-8. [DOI] [PubMed] [Google Scholar]
  8. Fischer D., Van der Weyden M. B., Snyderman R., Kelley W. N. A role for adenosine deaminase in human monocyte maturation. J Clin Invest. 1976 Aug;58(2):399–407. doi: 10.1172/JCI108484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gurd J. W., Evans W. H. Distribution of liver plasma membrane 5' nucleotidase as indicated by its reaction with anti-plasma membrane serum. Arch Biochem Biophys. 1974 Sep;164(1):305–311. doi: 10.1016/0003-9861(74)90035-6. [DOI] [PubMed] [Google Scholar]
  10. Harb J., Meflah K., Duflos Y., Bernard S. Purification and properties of bovine liver plasma membrane 5' nucleotidase. Eur J Biochem. 1983 Dec 1;137(1-2):131–138. doi: 10.1111/j.1432-1033.1983.tb07806.x. [DOI] [PubMed] [Google Scholar]
  11. Hayman M. J., Crumpton M. J. Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin. Biochem Biophys Res Commun. 1972 May 26;47(4):923–930. doi: 10.1016/0006-291x(72)90581-5. [DOI] [PubMed] [Google Scholar]
  12. Jones D. H., Matus A. I. Isolation of synaptic plasma membrane from brain by combined flotation-sedimentation density gradient centrifugation. Biochim Biophys Acta. 1974 Aug 9;356(3):276–287. doi: 10.1016/0005-2736(74)90268-5. [DOI] [PubMed] [Google Scholar]
  13. Kidwai A. M., Radcliffe M. A., Lee E. Y., Daniel E. E. Isolation and properties of skeletal muscle plasma membrane. Biochim Biophys Acta. 1973 Mar 29;298(3):593–607. doi: 10.1016/0005-2736(73)90076-x. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  16. Matsuura S., Eto S., Kato K., Tashiro Y. Ferritin immunoelectron microscopic localization of 5'-nucleotidase on rat liver cell surface. J Cell Biol. 1984 Jul;99(1 Pt 1):166–173. doi: 10.1083/jcb.99.1.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meflah K., Harb J., Duflos Y., Bernard S. 5'-Nucleotidase from bovine caudate nucleus synaptic plasma membranes: specificity for substrates and cations; study of the carbohydrate moiety by glycosidases. J Neurochem. 1984 Apr;42(4):1107–1115. doi: 10.1111/j.1471-4159.1984.tb12717.x. [DOI] [PubMed] [Google Scholar]
  18. Méflah K., Bernard S., Massoulié J. Interactions with lectins indicate differences in the carbohydrate composition of the membrane-bound enzymes acetylcholinesterase and 5'-nucleotidase in different cell types. Biochimie. 1984 Jan;66(1):59–69. doi: 10.1016/0300-9084(84)90192-5. [DOI] [PubMed] [Google Scholar]
  19. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  20. Riemer B. L., Widnell C. C. The demonstration of a specific 5'-nucleotidase activity in rat tissues. Arch Biochem Biophys. 1975 Nov;171(1):343–347. doi: 10.1016/0003-9861(75)90041-7. [DOI] [PubMed] [Google Scholar]
  21. Stanley K. K., Burke B., Pitt T., Siddle K., Luzio J. P. Localisation of 5'-nucleotidase in a rat liver cell line using a monoclonal antibody and indirect immunofluorescent labelling. Exp Cell Res. 1983 Mar;144(1):39–46. doi: 10.1016/0014-4827(83)90439-1. [DOI] [PubMed] [Google Scholar]
  22. Stanley K. K., Edwards M. R., Luzio J. P. Subcellular distribution and movement of 5'-nucleotidase in rat cells. Biochem J. 1980 Jan 15;186(1):59–69. doi: 10.1042/bj1860059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ternynck T., Avrameas S. Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde. FEBS Lett. 1972 Jun 1;23(1):24–28. doi: 10.1016/0014-5793(72)80274-6. [DOI] [PubMed] [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Trams E. G., Lauter C. J. On the sidedness of plasma membrane enzymes. Biochim Biophys Acta. 1974 Apr 29;345(2):180–197. doi: 10.1016/0005-2736(74)90257-0. [DOI] [PubMed] [Google Scholar]
  26. Zachowski A., Simonin G., Aubry J., Pommier P., Singh S. N., Potter M., Paraf A. Plasma membrane enzymes in BALB/c lymphomas with either T or B cell properties, I. 5'-Nucleotidase. J Recept Res. 1981;2(1):97–118. doi: 10.3109/10799898109038800. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES