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. 1986 Jan 1;233(1):207–213. doi: 10.1042/bj2330207

Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum.

G L Francis, L C Read, F J Ballard, C J Bagley, F M Upton, P M Gravestock, J C Wallace
PMCID: PMC1153005  PMID: 3954725

Abstract

Growth-promoting activity in bovine colostrum has been detected as the capacity to stimulate protein synthesis in L6 myoblasts. By using this assay as a measure of bioactivity, a growth factor has been purified to near homogeneity from centrifuged colostrum by a series of steps including acid extraction, chromatography on sulphopropyl-Sephadex, followed by adsorption to, and elution from, C18 columns using acetonitrile and propan-1-ol gradients. The purified growth factor has a low solubility at neutral and alkaline pH and has an Mr of 7800 by gel-permeation chromatography. Sequence analysis of the first 30 amino acids from the N-terminus indicated complete identity in this region with human insulin-like growth factor-1. Accordingly we conclude that the purified growth factor is bovine insulin-like growth factor-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballard F. J., Nield M. K., Francis G. L., Dahlenburg G. W., Wallace J. C. The relationship between the insulin content and inhibitory effects of bovine colostrum on protein breakdown in cultured cells. J Cell Physiol. 1982 Mar;110(3):249–254. doi: 10.1002/jcp.1041100305. [DOI] [PubMed] [Google Scholar]
  2. Ballard F. J., Read L. C., Francis G. L., Bagley C. J., Wallace J. C. Binding properties and biological potencies of insulin-like growth factors in L6 myoblasts. Biochem J. 1986 Jan 1;233(1):223–230. doi: 10.1042/bj2330223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ballard F. J. Regulation of protein accumulation in cultured cells. Biochem J. 1982 Nov 15;208(2):275–287. doi: 10.1042/bj2080275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baxter R. C., Zaltsman Z., Turtle J. R. Immunoreactive somatomedin-C/insulin-like growth factor I and its binding protein in human milk. J Clin Endocrinol Metab. 1984 Jun;58(6):955–959. doi: 10.1210/jcem-58-6-955. [DOI] [PubMed] [Google Scholar]
  5. Beardmore J. M., Lewis-Jones D. I., Richards R. C. Urogastrone and lactose concentrations in precolostrum, colostrum, and milk. Pediatr Res. 1983 Oct;17(10):825–828. doi: 10.1203/00006450-198310000-00012. [DOI] [PubMed] [Google Scholar]
  6. Blundell T. L., Bedarkar S., Humbel R. E. Tertiary structures, receptor binding, and antigenicity of insulinlike growth factors. Fed Proc. 1983 Jun;42(9):2592–2597. [PubMed] [Google Scholar]
  7. Brignon G., Ribadeau Dumas B., Mercier J. C., Pelissier J. P., Das B. C. Complete amino acid sequence of bovine alphaS2-casein. FEBS Lett. 1977 Apr 15;76(2):274–279. doi: 10.1016/0014-5793(77)80167-1. [DOI] [PubMed] [Google Scholar]
  8. Brown K. D., Blakeley D. M. Cell growth-promoting activity in mammary secretions of the goat, cow and sheep. Br Vet J. 1983 Jan-Feb;139(1):68–78. doi: 10.1016/s0007-1935(17)30594-8. [DOI] [PubMed] [Google Scholar]
  9. Brown K. D., Blakeley D. M. Partial purification and characterization of a growth factor present in goat's colostrum. Similarities with platelet-derived growth factor. Biochem J. 1984 Apr 15;219(2):609–617. doi: 10.1042/bj2190609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Carpenter G. Epidermal growth factor is a major growth-promoting agent in human milk. Science. 1980 Oct 10;210(4466):198–199. doi: 10.1126/science.6968093. [DOI] [PubMed] [Google Scholar]
  11. Chen R. F. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967 Jan 25;242(2):173–181. [PubMed] [Google Scholar]
  12. Gunn J. M., Bodner J. B., Knowles S. E., Ballard F. J. Inhibition of protein breakdown by epidermal growth factor in IMR90 human fibroblasts and other mammalian cell lines. Biochem J. 1983 Jan 15;210(1):251–258. doi: 10.1042/bj2100251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hunkapiller M. W., Hewick R. M., Dreyer W. J., Hood L. E. High-sensitivity sequencing with a gas-phase sequenator. Methods Enzymol. 1983;91:399–413. doi: 10.1016/s0076-6879(83)91038-8. [DOI] [PubMed] [Google Scholar]
  14. Klagsbrun M. Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture. J Cell Biol. 1980 Mar;84(3):808–814. doi: 10.1083/jcb.84.3.808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klagsbrun M. Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5057–5061. doi: 10.1073/pnas.75.10.5057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Klapper D. G., Svoboda M. E., Van Wyk J. J. Sequence analysis of somatomedin-C: confirmation of identity with insulin-like growth factor I. Endocrinology. 1983 Jun;112(6):2215–2217. doi: 10.1210/endo-112-6-2215. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Marquardt H., Todaro G. J., Henderson L. E., Oroszlan S. Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II. J Biol Chem. 1981 Jul 10;256(13):6859–6865. [PubMed] [Google Scholar]
  19. Moses A. C., Nissley S. P., Short P. A., Rechler M. M., Podskalny J. M. Purification and characterization of multiplication-stimulating activity. Insulin-like growth factors purified from rat-liver-cell-conditioned medium. Eur J Biochem. 1980 Jan;103(2):387–400. doi: 10.1111/j.1432-1033.1980.tb04325.x. [DOI] [PubMed] [Google Scholar]
  20. Read L. C., Ballard F. J., Francis G. L., Baxter R. C., Bagley C. J., Wallace J. C. Comparative binding of bovine, human and rat insulin-like growth factors to membrane receptors and to antibodies against human insulin-like growth factor-1. Biochem J. 1986 Jan 1;233(1):215–221. doi: 10.1042/bj2330215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Read L. C., Upton F. M., Francis G. L., Wallace J. C., Dahlenberg G. W., Ballard F. J. Changes in the growth-promoting activity of human milk during lactation. Pediatr Res. 1984 Feb;18(2):133–139. doi: 10.1203/00006450-198402000-00004. [DOI] [PubMed] [Google Scholar]
  22. Rinderknecht E., Humbel R. E. Polypeptides with nonsuppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization, and some biological properties of forms I and II. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2365–2369. doi: 10.1073/pnas.73.7.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rinderknecht E., Humbel R. E. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem. 1978 Apr 25;253(8):2769–2776. [PubMed] [Google Scholar]
  24. Rubin J. S., Mariz I., Jacobs J. W., Daughaday W. H., Bradshaw R. A. Isolation and partial sequence analysis of rat basic somatomedin. Endocrinology. 1982 Mar;110(3):734–740. doi: 10.1210/endo-110-3-734. [DOI] [PubMed] [Google Scholar]
  25. Sereni A., Baserga R. Routine growth of cell lines in medium supplemented with milk instead of serum. Cell Biol Int Rep. 1981 Apr;5(4):339–345. doi: 10.1016/0309-1651(81)90003-5. [DOI] [PubMed] [Google Scholar]
  26. Shing Y. W., Klagsbrun M. Human and bovine milk contain different sets of growth factors. Endocrinology. 1984 Jul;115(1):273–282. doi: 10.1210/endo-115-1-273. [DOI] [PubMed] [Google Scholar]
  27. Steimer K. S., Klagsbrun M. Serum-free growth of normal and transformed fibroblasts in milk: differential requirements for fibronectin. J Cell Biol. 1981 Feb;88(2):294–300. doi: 10.1083/jcb.88.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Svoboda M. E., Van Wyk J. J., Klapper D. G., Fellows R. E., Grissom F. E., Schlueter R. J. Purification of somatomedin-C from human plasma: chemical and biological properties, partial sequence analysis, and relationship to other somatomedins. Biochemistry. 1980 Feb 19;19(4):790–797. doi: 10.1021/bi00545a027. [DOI] [PubMed] [Google Scholar]
  29. Williams P. F., Turtle J. R. Purification of the insulin receptor from human placental membranes. Biochim Biophys Acta. 1979 Aug 28;579(2):367–374. doi: 10.1016/0005-2795(79)90064-3. [DOI] [PubMed] [Google Scholar]

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