Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1986 Feb 1;233(3):773–777. doi: 10.1042/bj2330773

A novel prenyltransferase from Paracoccus denitrificans.

K Ishii, H Sagami, K Ogura
PMCID: PMC1153098  PMID: 3707524

Abstract

A new polyprenyltransferase catalysing the formation of Z-double bonds was found and partially purified from extracts of Paracoccus denitrificans. The enzyme catalysed a consecutive condensation of isopentenyl diphosphate with EE-farnesyl diphosphate as a primer to produce EE-farnesyl-all-Z-hexaprenyl diphosphate (ZE-mixed nonaprenyl diphosphate) as the final product. Not only EE-farnesyl diphosphate but also neryl diphosphate, ZE-farnesyl diphosphate, ZEE-geranylgeranyl diphosphate and ZZEE-pentaprenyl diphosphate were all accepted as substrates. This polyprenyltransferase required detergent such as Triton X-100 for its catalytic activity. The formation of ZE-mixed undecaprenyl diphosphate, which is well known as the precursor of the bacterial sugar-carrier lipid, was not detected in extracts of this bacterium.

Full text

PDF
773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen C. M., Keenan M. V., Sack J. Lactobacillus plantarum undecaprenyl pyrophosphate synthetase: purification and reaction requirements. Arch Biochem Biophys. 1976 Jul;175(1):236–248. doi: 10.1016/0003-9861(76)90504-x. [DOI] [PubMed] [Google Scholar]
  2. Baba T., Allen C. M. Prenyl transferases from Micrococcus luteus: characterization of undecaprenyl pyrophosphate synthetase. Arch Biochem Biophys. 1980 Apr 1;200(2):474–484. doi: 10.1016/0003-9861(80)90379-3. [DOI] [PubMed] [Google Scholar]
  3. Fujii H., Koyama T., Ogura K. Efficient enzymatic hydrolysis of polyprenyl pyrophosphates. Biochim Biophys Acta. 1982 Sep 14;712(3):716–718. [PubMed] [Google Scholar]
  4. Fujii H., Koyama T., Ogura K. Hexaprenyl pyrophosphate synthetase from Micrococcus luteus B-P 26. Separation of two essential components. J Biol Chem. 1982 Dec 25;257(24):14610–14612. [PubMed] [Google Scholar]
  5. Fujii H., Sagami H., Koyama T., Ogura K., Seto S., Baba T., Allen C. M. Variable product specificity of solanesyl pyrophosphate synthetase. Biochem Biophys Res Commun. 1980 Oct 31;96(4):1648–1653. doi: 10.1016/0006-291x(80)91363-7. [DOI] [PubMed] [Google Scholar]
  6. Imai K., Asano A., Sato R. Oxidative phosphorylation in Micrococcus denitrificans. IV. Further characterization of electron-transfer pathway and phosphorylation activity in NADH oxidation. J Biochem. 1968 Feb;63(2):207–218. doi: 10.1093/oxfordjournals.jbchem.a128763. [DOI] [PubMed] [Google Scholar]
  7. KANDUTSCH A. A., PAULUS H., LEVIN E., BLOCH K. PURIFICATION OF GERANYLGERANYL PYROPHOSPHATE SYNTHETASE FROM MICROCOCCUS LYSODEIKTICUS. J Biol Chem. 1964 Aug;239:2507–2515. [PubMed] [Google Scholar]
  8. Kurokawa T., Ogura K., Seto S. Formation of polyprenyl phosphates by a cell-free enzyme of Micrococcus lysodeikticus. Biochem Biophys Res Commun. 1971 Oct 1;45(1):251–257. doi: 10.1016/0006-291x(71)90077-5. [DOI] [PubMed] [Google Scholar]
  9. Sagami H., Ogura K., Seto S., Kurokawa T. A new prenyltransferase from Micrococcus lysodeikticus. Biochem Biophys Res Commun. 1978 Nov 29;85(2):572–578. doi: 10.1016/0006-291x(78)91201-9. [DOI] [PubMed] [Google Scholar]
  10. Sagami H., Ogura K., Seto S. Solanesyl pyrophosphate synthetase from Micrococcus lysodeikticus. Biochemistry. 1977 Oct 18;16(21):4616–4622. doi: 10.1021/bi00640a014. [DOI] [PubMed] [Google Scholar]
  11. Takahashi I., Ogura K. Farnesyl pyrophosphate synthetase from Bacillus subtilis. J Biochem. 1981 May;89(5):1581–1587. doi: 10.1093/oxfordjournals.jbchem.a133352. [DOI] [PubMed] [Google Scholar]
  12. Takahashi I., Ogura K. Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase. J Biochem. 1982 Nov;92(5):1527–1537. doi: 10.1093/oxfordjournals.jbchem.a134077. [DOI] [PubMed] [Google Scholar]
  13. Takahashi I., Ogura K., Seto S. Heptaprenyl pyrophosphate synthetase from Bacillus subtilis. J Biol Chem. 1980 May 25;255(10):4539–4543. [PubMed] [Google Scholar]
  14. Wilkinson B. J., Morman M. R., White D. C. Phospholipid composition and metabolism of Micrococcus denitrificans. J Bacteriol. 1972 Dec;112(3):1288–1294. doi: 10.1128/jb.112.3.1288-1294.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES