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. 1986 Feb 1;233(3):921–924. doi: 10.1042/bj2330921

Removal of haem from lipids extracted from intact erythrocytes with particular reference to polyphosphoinositides.

S Christensen
PMCID: PMC1153119  PMID: 3010947

Abstract

With the use of a modified acid version of a current lipid-extraction technique [Bligh & Dyer (1959) Can. J. Biochem. Physiol. 37, 911-917], 92% of phosphatidylinositol 4,5-bisphosphate obtained by means of three sequential extractions from intact human erythrocytes was obtained during the first one. Some 95% of the haem co-extracted with the lipids could then be removed, with a maximal loss of polyphosphoinositides of less than 3%. About 58 nmol of phosphatidylinositol 4,5-bisphosphate was found per ml of erythrocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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