Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Jun 15;212(3):783–790. doi: 10.1042/bj2120783

Changes in the cytochrome composition of Rhodopseudomonas sphaeroides grown aerobically, photosynthetically and on dimethyl sulphoxide.

J A Ward, C N Hunter, O T Jones
PMCID: PMC1153155  PMID: 6603837

Abstract

Several strains and mutants of Rhodopseudomonas sphaeroides can be grown anaerobically in the dark in the presence of dimethyl sulphoxide as an electron acceptor. During adaptation to this fermentative mode of growth, two major c-type cytochromes are synthesized, one with Mr 45 000 and the second with Mr 20 000 and a midpoint potential of +120 mV. These cytochromes are barely detectable in membranes prepared from cells grown in aerobic or photosynthetic conditions. An electrophoretic method is presented for the detection of the b-type and c-type cytochromes of pigmented or unpigmented membranes. The method resolves three b-type cytochromes and four c-type cytochromes in membranes from aerobically and photosynthetically grown cells.

Full text

PDF
783

Images in this article

787Fig. 4.
on p.787
788Fig. 5.
on p.788

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett J., Hunter C. N., Jones O. T. Properties of a cytochrome c-enriched light particulate fraction isolated from the photosynthetic bacterium Rhodopseudomonas spheroides. Biochem J. 1978 Jul 15;174(1):267–275. doi: 10.1042/bj1740267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Broglie R. M., Hunter C. N., Delepelaire P., Niederman R. A., Chua N. H., Clayton R. K. Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis. Proc Natl Acad Sci U S A. 1980 Jan;77(1):87–91. doi: 10.1073/pnas.77.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLAYTON R. K. TOWARD THE ISOLATION OF A PHOTOCHEMICAL REACTION CENTER IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1963 Nov 29;75:312–323. doi: 10.1016/0006-3002(63)90618-8. [DOI] [PubMed] [Google Scholar]
  4. Castelfranco P. A., Jones O. T. Protoheme turnover and chlorophyll synthesis in greening barley tissue. Plant Physiol. 1975 Mar;55(3):485–490. doi: 10.1104/pp.55.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cross A. R., Jones O. T., Harper A. M., Segal A. W. Oxidation-reduction properties of the cytochrome b found in the plasma-membrane fraction of human neutrophils. A possible oxidase in the respiratory burst. Biochem J. 1981 Feb 15;194(2):599–606. doi: 10.1042/bj1940599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Delepelaire P., Chua N. H. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):111–115. doi: 10.1073/pnas.76.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gabellini N., Bowyer J. R., Hurt E., Melandri B. A., Hauska G. A cytochrome b/c1 complex with ubiquinol--cytochrome c2 oxidoreductase activity from Rhodopseudomonas sphaeroides GA. Eur J Biochem. 1982 Aug;126(1):105–111. doi: 10.1111/j.1432-1033.1982.tb06753.x. [DOI] [PubMed] [Google Scholar]
  8. Hendry G. A., Houghton J. D., Jones O. T. The cytochromes in microsomal fractions of germinating mung beans. Biochem J. 1981 Mar 15;194(3):743–751. doi: 10.1042/bj1940743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jones M. S., Jones O. T. Ferrochelatase of Rhodopseudomonas spheroides. Biochem J. 1970 Sep;119(3):453–462. doi: 10.1042/bj1190453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Niederman R. A., Mallon D. E., Langan J. J. Membranes of Rhodopseudomonas sphaeroides. IV. Assembly of chromatophores in low-aeration cell suspensions. Biochim Biophys Acta. 1976 Aug 13;440(2):429–447. doi: 10.1016/0005-2728(76)90076-1. [DOI] [PubMed] [Google Scholar]
  11. ORLANDO J. A. Rhodopseudomonas spheroides-cytochrome-553. Biochim Biophys Acta. 1962 Feb 26;57:373–375. doi: 10.1016/0006-3002(62)91133-2. [DOI] [PubMed] [Google Scholar]
  12. Prince R. C., Cogdell R. J., Crofts A. R. The photo-oxidation of horse heart cytochrome c and native cytochrome c2 by reaction centres from Rhodopseudomonas spheroides R26. Biochim Biophys Acta. 1974 Apr 23;347(1):1–13. doi: 10.1016/0005-2728(74)90194-7. [DOI] [PubMed] [Google Scholar]
  13. SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
  14. Saunders V. A., Jones O. T. Detection of two further beta-type cytochromes in Rhodopseudomonas spheroides. Biochim Biophys Acta. 1975 Aug 11;396(2):220–228. doi: 10.1016/0005-2728(75)90036-5. [DOI] [PubMed] [Google Scholar]
  15. Thomas P. E., Ryan D., Levin W. An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Anal Biochem. 1976 Sep;75(1):168–176. doi: 10.1016/0003-2697(76)90067-1. [DOI] [PubMed] [Google Scholar]
  16. Uffen R. L., Wolfe R. S. Anaerobic growth of purple nonsulfur bacteria under dark conditions. J Bacteriol. 1970 Oct;104(1):462–472. doi: 10.1128/jb.104.1.462-472.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wood P. M. Do photosynthetic bacteria contain cytochrome c1? Biochem J. 1980 Sep 1;189(3):385–391. doi: 10.1042/bj1890385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wood P. M. The redox potential for dimethyl sulphoxide reduction to dimethyl sulphide: evaluation and biochemical implications. FEBS Lett. 1981 Feb 9;124(1):11–14. doi: 10.1016/0014-5793(81)80042-7. [DOI] [PubMed] [Google Scholar]
  19. Yen H. C., Marrs B. Growth of Rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide. Arch Biochem Biophys. 1977 Jun;181(2):411–418. doi: 10.1016/0003-9861(77)90246-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES