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. 1984 Jan 1;217(1):331–334. doi: 10.1042/bj2170331

Accessibility of DNA-epoxycellulose to sequence-specific DNA-binding proteins.

P A Lazo
PMCID: PMC1153215  PMID: 6320804

Abstract

Phage lambda DNA was covalently coupled to epoxy-activated cellulose to form a stable DNA-cellulose matrix for affinity chromatography of sequence-specific DNA-binding proteins. The accessibility of three specific six-base sequences, GGATCC (BamHI), GAATTC (EcoRI) and AAGCTT (HindIII) was studied quantitatively and qualitatively by restriction analysis followed by labelling of their recessed ends. All sites are randomly accessible. The site accessibility is variable, BamHI greater than HindIII greater than EcoRI, and within the range 20-100% depending on base composition and internal structure of the sequence. DNA-epoxycellulose, because of its high efficiency of coupling, capacity, stability and accessibility, can be of great help in the isolation and characterization of sequence-specific DNA-binding proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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