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. 1984 Feb 1;217(3):855–857. doi: 10.1042/bj2170855

Effects of glucagon in vivo on the N-acetylglutamate, glutamate and glutamine contents of rat liver.

J M Staddon, N M Bradford, J D McGivan
PMCID: PMC1153292  PMID: 6143551

Abstract

Glucagon injected into rats caused an increase in liver N-acetylglutamate content, and coincidentally the glutamate content decreased. The liver glutamine content decreased only after 10 min, consistent with an activation of glutaminase after a lag. These observations indicate that the increased N-acetylglutamate content was not due to an increase in glutamate content caused by an activation of glutaminase (EC 3.5.1.2).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brosnan J. T., Williamson D. H. Mechanisms for the formation of alanine and aspartate on rat liver in vivo after administration of ammonium chloride. Biochem J. 1974 Mar;138(3):453–462. doi: 10.1042/bj1380453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cook G. A., Nielsen R. C., Hawkins R. A., Mehlman M. A., Lakshmanan M. R., Veech R. L. Effect of glucagon on hepatic malonyl coenzyme A concentration and on lipid synthesis. J Biol Chem. 1977 Jun 25;252(12):4421–4424. [PubMed] [Google Scholar]
  3. Gomez M., Jordá A., Cabo J., Grisolía S. Effect of starvation on the N-acetylglutamate system of rat liver. FEBS Lett. 1983 May 30;156(1):119–122. doi: 10.1016/0014-5793(83)80260-9. [DOI] [PubMed] [Google Scholar]
  4. Hensgens H. E., Verhoeven A. J., Meijer A. J. The relationship between intramitochondrial N-acetylglutamate and activity of carbamoyl-phosphate synthetase (ammonia). The effect of glucagon. Eur J Biochem. 1980;107(1):197–205. doi: 10.1111/j.1432-1033.1980.tb04640.x. [DOI] [PubMed] [Google Scholar]
  5. Joseph S. K., McGivan J. D. The effect of ammonium chloride and glucagon on the metabolism of glutamine in isolated liver cells from starved rats. Biochim Biophys Acta. 1978 Sep 21;543(1):16–28. doi: 10.1016/0304-4165(78)90450-6. [DOI] [PubMed] [Google Scholar]
  6. Kovacevic Z., McGivan J. D. Mitochondrial metabolism of glutamine and glutamate and its physiological significance. Physiol Rev. 1983 Apr;63(2):547–605. doi: 10.1152/physrev.1983.63.2.547. [DOI] [PubMed] [Google Scholar]
  7. McGivan J. D., Bradford N. M., Mendes-Mourão J. The regulation of carbamoyl phosphate synthase activity in rat liver mitochondria. Biochem J. 1976 Feb 15;154(2):415–421. doi: 10.1042/bj1540415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meijer A. J., van Woerkom G. M. Control of the rate of citrulline synthesis by short-term changes in N-acetylglutamate levels in isolated rat-liver mitochondria. FEBS Lett. 1978 Feb 1;86(1):117–121. doi: 10.1016/0014-5793(78)80111-2. [DOI] [PubMed] [Google Scholar]
  9. Raijman L., Jones M. E. Purification, composition, and some properties of rat liver carbamyl phosphate synthetase (ammonia). Arch Biochem Biophys. 1976 Jul;175(1):270–278. doi: 10.1016/0003-9861(76)90508-7. [DOI] [PubMed] [Google Scholar]
  10. Saheki T., Ohkubo T., Katsunuma T. Regulation of urea synthesis in rat liver. Increase in the concentrations of ornithine and acetylglutamate in rat liver in response to urea synthesis stimulated by the injection of an ammonium salt. J Biochem. 1978 Dec;84(6):1423–1430. doi: 10.1093/oxfordjournals.jbchem.a132264. [DOI] [PubMed] [Google Scholar]
  11. Shigesada K., Tatibana M. Role of acetylglutamate in ureotelism. I. Occurrence and biosynthesis of acetylglutamate in mouse and rat tissues. J Biol Chem. 1971 Sep 25;246(18):5588–5595. [PubMed] [Google Scholar]
  12. Siess E. A., Brocks D. G., Lattke H. K., Wieland O. H. Effect of glucagon on metabolite compartmentation in isolated rat liver cells during gluconeogenesis from lactate. Biochem J. 1977 Aug 15;166(2):225–235. doi: 10.1042/bj1660225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Siess E. A., Wieland O. H. Glucagon-induced stimulation of 2-oxoglutarate metabolism in mitochondria from rat liver. FEBS Lett. 1978 Sep 15;93(2):301–306. doi: 10.1016/0014-5793(78)81126-0. [DOI] [PubMed] [Google Scholar]
  14. Stewart P. M., Walser M. Short term regulation of ureagenesis. J Biol Chem. 1980 Jun 10;255(11):5270–5280. [PubMed] [Google Scholar]
  15. Williamson D. H., Lund P., Krebs H. A. The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J. 1967 May;103(2):514–527. doi: 10.1042/bj1030514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamazaki R. K., Graetz G. S. Glucagon stimulation of citrulline formation in isolated hepatic mitochondria. Arch Biochem Biophys. 1977 Jan 15;178(1):19–25. doi: 10.1016/0003-9861(77)90166-7. [DOI] [PubMed] [Google Scholar]

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