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. 1984 Feb 15;218(1):113–118. doi: 10.1042/bj2180113

Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii.

A J Balmforth, A Thomson
PMCID: PMC1153313  PMID: 6712607

Abstract

Glyoxylate dehydrogenase (glyoxylate:NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.

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Selected References

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