Abstract
The Michaelis-Menten equation for the utilization of competing substrates was applied to the uptake of 2-deoxy[3H]glucose into the mammary gland of anaesthetized lactating rats. Intracellular water was calculated from total tissue water and sucrose space. Fed rats had a mean transport capacity of 2.2 mumol/min per g of tissue, giving an actual glucose transport in vivo of 1.1 mumol/min per g. Transport decreased by 90% on overnight starvation and returned to normal by 2 h of re-feeding. Similar changes were observed in the 1 min or 5 min transport of circulating 3-O-methylglucose. Transport of 3-O-methylglucose in starved rats was restored towards normal by insulin. In fed rats it increased between parturition and day 12 of lactation. The findings support the proposal that transport is a rate-limiting factor in the mammary utilization of carbohydrate.
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Agius L., Williamson D. H. Rapid inhibition of lipogenesis in vivo in lactating rat mammary gland by medium- or long-chain triacylglycerols and partial reversal by insulin. Biochem J. 1980 Oct 15;192(1):361–364. doi: 10.1042/bj1920361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Amato P. A., Loizzi R. F. The effects of cytochalasin B on glucose transport and lactose synthesis in lactating mammary gland slices. Eur J Cell Biol. 1979 Dec;20(2):150–155. [PubMed] [Google Scholar]
- CRONE C. THE PERMEABILITY OF CAPILLARIES IN VARIOUS ORGANS AS DETERMINED BY USE OF THE 'INDICATOR DIFFUSION' METHOD. Acta Physiol Scand. 1963 Aug;58:292–305. doi: 10.1111/j.1748-1716.1963.tb02652.x. [DOI] [PubMed] [Google Scholar]
- Chatwin A. L., Linzell J. L., Setchell B. P. Cardiovascular changes during lactation in the rat. J Endocrinol. 1969 Jun;44(2):247–254. doi: 10.1677/joe.0.0440247. [DOI] [PubMed] [Google Scholar]
- Crone C. Facilitated transfer of glucose from blood into brain tissue. J Physiol. 1965 Nov;181(1):103–113. doi: 10.1113/jphysiol.1965.sp007748. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eidelberg E., Fishman J., Hams M. L. Penetration of sugars across the blood-brain barrier. J Physiol. 1967 Jul;191(1):47–57. doi: 10.1113/jphysiol.1967.sp008236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elkin A. R., Kuhn N. J. Aerobic lactate production by mammary tissue. Biochem J. 1975 Jan;146(1):273–275. doi: 10.1042/bj1460273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FISHMAN R. A. CARRIER TRANSPORT OF GLUCOSE BETWEEN BLOOD AND CEREBROSPINAL FLUID. Am J Physiol. 1964 Apr;206:836–844. doi: 10.1152/ajplegacy.1964.206.4.836. [DOI] [PubMed] [Google Scholar]
- Faulkner A., Chaiyabutr N., Peaker M., Carrick D. T., Kuhn N. J. Metabolic significance of milk glucose. J Dairy Res. 1981 Feb;48(1):51–56. doi: 10.1017/s0022029900021440. [DOI] [PubMed] [Google Scholar]
- Foley J. E., Foley R., Gliemann J. Rate-limiting steps of 2-deoxyglucose uptake in rat adipocytes. Biochim Biophys Acta. 1980 Jul;599(2):689–698. doi: 10.1016/0005-2736(80)90210-2. [DOI] [PubMed] [Google Scholar]
- Goresky C. A., Nadeau B. E. Uptake of materials by the intact liver. The exchange of glucose across the cell membranes. J Clin Invest. 1974 Feb;53(2):634–646. doi: 10.1172/JCI107598. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn N. J., White A. Milk glucose as an index of the intracellular glucose concentration of rat mammary gland. Biochem J. 1975 Oct;152(1):153–155. doi: 10.1042/bj1520153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Comparison of glucose metabolism in the lactating mammary gland of the rat in vivo and in vitro. Effects of starvation, prolactin or insulin deficiency. Biochem J. 1977 Apr 15;164(1):153–159. doi: 10.1042/bj1640153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Control of glucose metabolism in isolated acini of the lactating mammary gland of the rat. The ability of glycerol to mimic some of the effects of insulin. Biochem J. 1977 Dec 15;168(3):465–474. doi: 10.1042/bj1680465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Effects of acetoacetate administration on glucose metabolism in mammary gland of fed lactating rats. Biochem J. 1977 Jun 15;164(3):749–752. doi: 10.1042/bj1640749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sokoloff L., Reivich M., Kennedy C., Des Rosiers M. H., Patlak C. S., Pettigrew K. D., Sakurada O., Shinohara M. The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem. 1977 May;28(5):897–916. doi: 10.1111/j.1471-4159.1977.tb10649.x. [DOI] [PubMed] [Google Scholar]
- Sutter-Dub M. T., Leclercq R., Sutter B. C., Jacquot R. Plasma glucose, progesterone and immunoreactive insulin levels in the lactating rat. Horm Metab Res. 1974 Jul;6(4):297–300. doi: 10.1055/s-0028-1093852. [DOI] [PubMed] [Google Scholar]
- Threadgold L. C., Coore H. G., Kuhn N. J. Monosaccharide transport into lactating-rat mammary acini. Biochem J. 1982 May 15;204(2):493–501. doi: 10.1042/bj2040493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilde C. J., Kuhn N. J. Lactose synthesis and the utilisation of glucose by rat mammary acini. Int J Biochem. 1981;13(3):311–316. doi: 10.1016/0020-711x(81)90083-5. [DOI] [PubMed] [Google Scholar]
- Wilde C. J., Kuhn N. J. Lactose synthesis in the rat, and the effects of litter size and malnutrition. Biochem J. 1979 Aug 15;182(2):287–294. doi: 10.1042/bj1820287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams T. F., Exton J. H., Park C. R., Regen D. M. Stereospecific transport of glucose in the perfused rat liver. Am J Physiol. 1968 Nov;215(5):1200–1209. doi: 10.1152/ajplegacy.1968.215.5.1200. [DOI] [PubMed] [Google Scholar]
- Williamson D. H., McKeown S. R., Ilic V. Interactions of glucose, acetoacetate and insulin in mammary-gland slices of lactating rats. Biochem J. 1975 Aug;150(2):145–152. doi: 10.1042/bj1500145. [DOI] [PMC free article] [PubMed] [Google Scholar]