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. 1984 Mar 1;218(2):625–628. doi: 10.1042/bj2180625

Specificity studies on the oligosaccharide neuraminidase of human fibroblasts.

K Mendla, M Cantz
PMCID: PMC1153381  PMID: 6424662

Abstract

Competition and thermal inactivation experiments with different potential natural substrates indicated that in homogenates of human fibroblasts one single enzyme is acting on both (alpha 2-3) and (alpha 2-6) sialosyl linkages of oligosaccharides and glycoproteins, but not of the ganglioside GM3. N-Acetylneuraminic and 2-deoxy-2,3-dehydro-N-acetylneuraminic acids are competitive inhibitors, whereas chondroitin 4-sulphate and the drug Suramin are potent inhibitors of undefined type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach G., Zeigler M., Schaap T., Kohn G. Mucolipidosis type IV: ganglioside sialidase deficiency. Biochem Biophys Res Commun. 1979 Oct 29;90(4):1341–1347. doi: 10.1016/0006-291x(79)91183-5. [DOI] [PubMed] [Google Scholar]
  2. Baenziger J. U., Fiete D. Structure of the complex oligosaccharides of fetuin. J Biol Chem. 1979 Feb 10;254(3):789–795. [PubMed] [Google Scholar]
  3. Baumkötter J., Cantz M. Decreased ganglioside neuraminidase activity in fibroblasts from mucopolysaccharidosis patients. Inhibition of the activity in vitro by sulfated glycosaminoglycans and other compounds. Biochim Biophys Acta. 1983 Dec 13;761(2):163–170. doi: 10.1016/0304-4165(83)90225-8. [DOI] [PubMed] [Google Scholar]
  4. Cantz M., Messer H. Oligosaccharide and ganglioside neuraminidase activities of mucolipidosis I (sialidosis) and mucolipidosis II (I-cell disease) fibroblasts. Eur J Biochem. 1979 Jun;97(1):113–118. doi: 10.1111/j.1432-1033.1979.tb13091.x. [DOI] [PubMed] [Google Scholar]
  5. Constantopoulos G., Rees S., Cragg B. G., Barranger J. A., Brady R. O. Experimental animal model for mucopolysaccharidosis: suramin-induced glycosaminoglycan and sphingolipid accumulation in the rat. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3700–3704. doi: 10.1073/pnas.77.6.3700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frisch A., Neufeld E. F. A rapid and sensitive assay for neuraminidase: application to cultured fibroblasts. Anal Biochem. 1979 May;95(1):222–227. doi: 10.1016/0003-2697(79)90209-4. [DOI] [PubMed] [Google Scholar]
  7. Gorham S. D., Cantz M. Arylsulphatase B, an exo-sulphatase for chondroitin 4-sulphate tetrasaccharide. Hoppe Seylers Z Physiol Chem. 1978 Dec;359(12):1811–1814. [PubMed] [Google Scholar]
  8. O'Brien J. S. Neuraminidase deficiency in the cherry red spot-myoclonus syndrome. Biochem Biophys Res Commun. 1977 Dec 21;79(4):1136–1141. doi: 10.1016/0006-291x(77)91124-x. [DOI] [PubMed] [Google Scholar]
  9. SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. doi: 10.1111/j.1471-4159.1963.tb08933.x. [DOI] [PubMed] [Google Scholar]
  10. Skoza L., Mohos S. Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de-O-acetylation. Biochem J. 1976 Dec 1;159(3):457–462. doi: 10.1042/bj1590457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spiro R. G., Bhoyroo V. D. Structure of the O-glycosidically linked carbohydrate units of fetuin. J Biol Chem. 1974 Sep 25;249(18):5704–5717. [PubMed] [Google Scholar]
  12. Strecker G., Michalski J. C. Biochemical basis of six different types of sialidosis. FEBS Lett. 1978 Jan 1;85(1):20–24. doi: 10.1016/0014-5793(78)81239-3. [DOI] [PubMed] [Google Scholar]
  13. Wenger D. A., Tarby T. J., Wharton C. Macular cherry-red spots and myoclonus with dementia: coexistent neuraminidase and beta-galactosidase deficiencies. Biochem Biophys Res Commun. 1978 May 30;82(2):589–595. doi: 10.1016/0006-291x(78)90915-4. [DOI] [PubMed] [Google Scholar]
  14. Zeigler M., Bach G. Cellular localization of neuraminidases in cultured human fibroblasts. Biochem J. 1981 Sep 15;198(3):505–508. doi: 10.1042/bj1980505. [DOI] [PMC free article] [PubMed] [Google Scholar]

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