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. 1984 Mar 15;218(3):983–985. doi: 10.1042/bj2180983

Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids.

J M Gutteridge
PMCID: PMC1153432  PMID: 6721843

Abstract

Copper(II) ions, in the presence of 1,10-phenanthroline, O2 and a reducing agent, degrade DNA with the release of thiobarbituric-acid-reactive material. This reaction, dependent on the formation of oxygen radicals, was made the basis of a sensitive and specific assay for loosely bound copper in body fluids. When applied to certain extracellular fluids, trace amounts of copper could be detected in the lower micromolar range.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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