Abstract
Methaemoglobin may be an important factor for initiation and development of lipid peroxidation in Cu(II)-treated red blood cells. It seems likely that the initiation of peroxidation by methaemoglobin is only possible if direct contact between haemoglobin molecule and the cell membrane is realized. In view of this, the binding of haemoglobin to the red-blood-cell membrane in the presence of CuCl2 was studied. It was found that the haemoglobin quenching of the fluorescence of 12-(9-anthroyl)stearic acid-labelled red-blood-cell membranes greatly increases in the presence of CuCl2. This effect is relatively independent of pH and the ionic strength of the medium, indicating that in this case the binding of haemoglobin is not electrostatic in nature. The haemoglobin quenching of the fluorescence of the inside-out and the right-side-out resealed ghosts were almost the same in the presence of CuCl2. This result suggests that, in the presence of ionic copper, both surfaces of the membrane possess approximately equal amounts of sites for the binding of haemoglobin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hochstein P., Kumar K. S., Forman S. J. Mechanisms of copper toxicity in red cells. Prog Clin Biol Res. 1978;21:669–686. [PubMed] [Google Scholar]
- Kumar K. S., Rowse C., Hochstein P. Copper-induced generation of superoxide in human red cell membrane. Biochem Biophys Res Commun. 1978 Jul 28;83(2):587–592. doi: 10.1016/0006-291x(78)91030-6. [DOI] [PubMed] [Google Scholar]
- Miura T., Ogiso T. Formation of denatured erythrocytes by exposure to a superoxide radical generating system of xanthine oxidase. Chem Pharm Bull (Tokyo) 1981 Jun;29(6):1730–1735. doi: 10.1248/cpb.29.1730. [DOI] [PubMed] [Google Scholar]
- Ribarov S. R., Benov L. C., Benchev I. C. The effect of lead on hemoglobin-catalyzed lipid peroxidation. Biochim Biophys Acta. 1981 Jun 23;664(3):453–459. doi: 10.1016/0005-2760(81)90123-5. [DOI] [PubMed] [Google Scholar]
- Ribarov S. R., Benov L. C. Relationship between the hemolytic action of heavy metals and lipid peroxidation. Biochim Biophys Acta. 1981 Feb 6;640(3):721–726. doi: 10.1016/0005-2736(81)90102-4. [DOI] [PubMed] [Google Scholar]
- Rifkind J. M. Copper and the autoxidation of hemoglobin. Biochemistry. 1974 Jun 4;13(12):2475–2481. doi: 10.1021/bi00709a003. [DOI] [PubMed] [Google Scholar]
- Shaklai N., Yguerabide J., Ranney H. M. Classification and localization of hemoglobin binding sites on the red blood cell membrane. Biochemistry. 1977 Dec 13;16(25):5593–5597. doi: 10.1021/bi00644a032. [DOI] [PubMed] [Google Scholar]
- Shaklai N., Yguerabide J., Ranney H. M. Interaction of hemoglobin with red blood cell membranes as shown by a fluorescent chromophore. Biochemistry. 1977 Dec 13;16(25):5585–5592. doi: 10.1021/bi00644a031. [DOI] [PubMed] [Google Scholar]
- Steck T. L., Kant J. A. Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes. Methods Enzymol. 1974;31:172–180. doi: 10.1016/0076-6879(74)31019-1. [DOI] [PubMed] [Google Scholar]
- TAPPEL A. L. Unsaturated lipide oxidation catalyzed by hematin compounds. J Biol Chem. 1955 Dec;217(2):721–733. [PubMed] [Google Scholar]
- WILLS E. D. MECHANISMS OF LIPID PEROXIDE FORMATION IN TISSUES. ROLE OF METALS AND HAEMATIN PROTEINS IN THE CATALYSIS OF THE OXIDATION UNSATURATED FATTY ACIDS. Biochim Biophys Acta. 1965 Apr 5;98:238–251. doi: 10.1016/0005-2760(65)90118-9. [DOI] [PubMed] [Google Scholar]
- Winterbourn C. C., Carrell R. W. Oxidation of human haemoglobin by copper. Mechanism and suggested role of the thiol group of residue beta-93. Biochem J. 1977 Jul 1;165(1):141–148. doi: 10.1042/bj1650141. [DOI] [PMC free article] [PubMed] [Google Scholar]