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. 1984 May 1;219(3):959–963. doi: 10.1042/bj2190959

Augmentation of dexamethasone induction of rat liver metallothionein by zinc.

S R Quinones, R J Cousins
PMCID: PMC1153568  PMID: 6743254

Abstract

The induction of liver metallothionein by dexamethasone in adrenalectomized rats was augmented by zinc administration. Metallothionein synthesis was increased in an additive manner with both zinc and dexamethasone compared with either treatment alone. Translational activity of polyribosomal metallothionein mRNA was also greater in zinc + dexamethasone-treated rats. Northern-blot analyses showed that dexamethasone increased these mRNA contents to a greater extent at the lower zinc dose, suggesting that the induction may be maximal at the higher zinc dose when combined with dexamethasone.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen R. D., Weser U. Partial purification, characterization and translation in vitro of rat liver metallothionein messenger ribonucleic acid. Biochem J. 1978 Dec 1;175(3):841–852. doi: 10.1042/bj1750841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bajwa W., Birnie G. D., Knowler J. T. Changes induced in rat liver polysomal polyadenylated RNAs by depletion of circulating glucocorticoids. Nucleic Acids Res. 1982 Jun 11;10(11):3541–3560. doi: 10.1093/nar/10.11.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bremner I., Davies N. T. The induction of metallothionein in rat liver by zinc injection and restriction of food intake. Biochem J. 1975 Sep;149(3):733–738. doi: 10.1042/bj1490733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Durnam D. M., Palmiter R. D. Transcriptional regulation of the mouse metallothionein-I gene by heavy metals. J Biol Chem. 1981 Jun 10;256(11):5712–5716. [PubMed] [Google Scholar]
  5. Durnam D. M., Perrin F., Gannon F., Palmiter R. D. Isolation and characterization of the mouse metallothionein-I gene. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511–6515. doi: 10.1073/pnas.77.11.6511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Etzel K. R., Cousins R. J. Hormonal regulation of liver metallothionein zinc: independent and synergistic action of glucagon and glucocorticoids. Proc Soc Exp Biol Med. 1981 Jun;167(2):233–236. doi: 10.3181/00379727-167-41155. [DOI] [PubMed] [Google Scholar]
  7. Etzel K. R., Shapiro S. G., Cousins R. J. Regulation of liver metallothionein and plasma zinc by the glucocorticoid dexamethasone. Biochem Biophys Res Commun. 1979 Aug 28;89(4):1120–1126. doi: 10.1016/0006-291x(79)92124-7. [DOI] [PubMed] [Google Scholar]
  8. Etzel K. R., Swerdel M. R., Swerdel J. N., Cousins R. J. Endotoxin-induced changes in copper and zinc metabolism in the Syrian hamster. J Nutr. 1982 Dec;112(12):2363–2373. doi: 10.1093/jn/112.12.2363. [DOI] [PubMed] [Google Scholar]
  9. Failla M. L., Cousins R. J. Zinc accumulation and metabolism in primary cultures of adult rat liver cells. Regulation by glucocorticoids. Biochim Biophys Acta. 1978 Oct 18;543(3):293–304. doi: 10.1016/0304-4165(78)90047-8. [DOI] [PubMed] [Google Scholar]
  10. Hager L. J., Palmiter R. D. Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids. Nature. 1981 May 28;291(5813):340–342. doi: 10.1038/291340a0. [DOI] [PubMed] [Google Scholar]
  11. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mayo K. E., Palmiter R. D. Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells. J Biol Chem. 1981 Mar 25;256(6):2621–2624. [PubMed] [Google Scholar]
  13. Mayo K. E., Warren R., Palmiter R. D. The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells. Cell. 1982 May;29(1):99–108. doi: 10.1016/0092-8674(82)90094-0. [DOI] [PubMed] [Google Scholar]
  14. McCormick C. C., Menard M. P., Cousins R. J. Induction of hepatic metallothionein by feeding zinc to rats of depleted zinc status. Am J Physiol. 1981 Apr;240(4):E414–E421. doi: 10.1152/ajpendo.1981.240.4.E414. [DOI] [PubMed] [Google Scholar]
  15. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oh S. H., Deagen J. T., Whanger P. D., Weswig P. H. Biological function of metallothionein. V. Its induction in rats by various stresses. Am J Physiol. 1978 Mar;234(3):E282–E285. doi: 10.1152/ajpendo.1978.234.3.E282. [DOI] [PubMed] [Google Scholar]
  17. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  18. Pavlakis G. N., Hamer D. H. Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus. Proc Natl Acad Sci U S A. 1983 Jan;80(2):397–401. doi: 10.1073/pnas.80.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ricciardi R. P., Miller J. S., Roberts B. E. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927–4931. doi: 10.1073/pnas.76.10.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Richards M. P., Cousins R. J. Mammalian zinc homeostasis: requirement for RNA and metallothionein synthesis. Biochem Biophys Res Commun. 1975 Jun 16;64(4):1215–1223. doi: 10.1016/0006-291x(75)90822-0. [DOI] [PubMed] [Google Scholar]
  21. Richards M. P., Cousins R. J. Metallothionein and its relationship to the metabolism of dietary zinc in rats. J Nutr. 1976 Nov;106(11):1591–1599. doi: 10.1093/jn/106.11.1591. [DOI] [PubMed] [Google Scholar]
  22. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  23. Shapiro S. G., Cousins R. J. Induction of rat liver metallothionein mRNA and its distribution between free and membrane-bound polyribosomes. Biochem J. 1980 Sep 15;190(3):755–764. doi: 10.1042/bj1900755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shapiro S. G., Squibb K. S., Markowitz L. A., Cousins R. J. Cell-free synthesis of metallothionein directed by rat liver polyadenylated messenger ribonucleic acid. Biochem J. 1978 Dec 1;175(3):833–840. doi: 10.1042/bj1750833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sobocinski P. Z., Canterbury W. J., Jr, Mapes C. A., Dinterman R. E. Involvement of hepatic metallothioneins in hypozincemia associated with bacterial infection. Am J Physiol. 1978 Apr;234(4):E399–E406. doi: 10.1152/ajpendo.1978.234.4.E399. [DOI] [PubMed] [Google Scholar]
  26. Squibb K. S., Cousins R. J., Feldman S. L. Control of zinc-thionein synthesis in rat liver. Biochem J. 1977 Apr 15;164(1):223–228. doi: 10.1042/bj1640223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]

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