Abstract
There are two possible contributions to the heterogeneity of mucus glycoproteins as observed in the analytical ultracentrifuge: firstly, from associative phenomena, and, secondly, from a polydisperse distribution of non-interacting species of different molecular masses. It is shown from the non-superposability of plots of point-weight-average relative molecular masses against concentration for differing initial cell-loading concentrations that polydispersity must be significant. It is further shown, by attempting to block any associative phenomena by competitive inhibition of potential sites for hydrophobic inter-particle interaction, that the observed heterogeneity is primarily a result of polydispersity and not a self-association.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bhaskar K. R., Donald A. S., Morgan W. T., Creeth J. C. The macromolecular properties of blood-group-specific glycoproteins. Characterization of a series of fractions obtained by solvent fractionation. Biochem J. 1974 Oct;143(1):159–170. doi: 10.1042/bj1430159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Creeth J. M., Bhaskar K. R., Horton J. R., Das I., Lopez-Vidriero M. T., Reid L. The separation and characterization of bronchial glycoproteins by density-gradient methods. Biochem J. 1977 Dec 1;167(3):557–569. doi: 10.1042/bj1670557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Creeth J. M., Harding S. E. A simple test for macromolecular heterogeneity in the analytical ultracentrifuge. Biochem J. 1982 Sep 1;205(3):639–641. doi: 10.1042/bj2050639. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Creeth J. M., Harding S. E. Some observations on a new type of point average molecular weight. J Biochem Biophys Methods. 1982 Dec;7(1):25–34. doi: 10.1016/0165-022x(82)90033-1. [DOI] [PubMed] [Google Scholar]
- Creeth J. M., Knight C. G. The macromolecular properties of blood-group substances. Sedimentation-velocity and viscosity measurements. Biochem J. 1967 Dec;105(3):1135–1145. doi: 10.1042/bj1051135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Creeth J. M. Molecular-weight determination in polydisperse non-ideal systems. Biochem Soc Trans. 1980 Oct;8(5):519–520. doi: 10.1042/bst0080519a. [DOI] [PubMed] [Google Scholar]
- Harding S. E., Creeth J. M. Polyelectrolyte behaviour in mucus glycoproteins. Biochim Biophys Acta. 1983 Jul 28;746(1-2):114–119. doi: 10.1016/0167-4838(83)90017-1. [DOI] [PubMed] [Google Scholar]
- Harding S. E., Rowe A. J., Creeth J. M. Further evidence for a flexible and highly expanded spheroidal model for mucus glycoproteins in solution. Biochem J. 1983 Mar 1;209(3):893–896. doi: 10.1042/bj2090893. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roark D. E., Yphantis D. A. Studies of self-associating systems by equilibrium ultracentrifugation. Ann N Y Acad Sci. 1969 Nov 7;164(1):245–278. doi: 10.1111/j.1749-6632.1969.tb14043.x. [DOI] [PubMed] [Google Scholar]
- Roussel P., Lamblin G., Degand P. Heterogeneity of the carbohydrate chains of sulfated bronchial glycoproteins isolated from a patient suffering from cystic fibrosis. J Biol Chem. 1975 Mar 25;250(6):2114–2122. [PubMed] [Google Scholar]
- Teller D. C. Characterization of proteins by sedimentation equilibrium in the analytical ultracentrifuge. Methods Enzymol. 1973;27:346–441. doi: 10.1016/s0076-6879(73)27017-9. [DOI] [PubMed] [Google Scholar]
- YPHANTIS D. A. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964 Mar;3:297–317. doi: 10.1021/bi00891a003. [DOI] [PubMed] [Google Scholar]