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. 1984 May 15;220(1):15–24. doi: 10.1042/bj2200015

Renal transport of neutral amino acids. Tubular localization of Na+-dependent phenylalanine- and glucose-transport systems.

U Kragh-Hansen, H Røigaard-Petersen, C Jacobsen, M I Sheikh
PMCID: PMC1153589  PMID: 6743259

Abstract

The transport properties for phenylalanine and glucose in luminal-membrane vesicles from outer cortex (pars convoluta) and outer medulla (pars recta) of rabbit kidney were studied by a spectrophotometric method. Uptake of phenylalanine as well as of glucose by the two types of membrane vesicles was found to be Na+-dependent, electrogenic and stereospecific. Na+-dependent transport of L-phenylalanine by outer-cortical membrane vesicles could be accounted for by one transport system (KA congruent to 1.5 mM). By contrast, in the outer-medullary preparation, L-phenylalanine transport occurred via two transport systems, namely a high-affinity system with K1A congruent to 0.33 mM and a low-affinity system with K2A congruent to 7 mM respectively. Na+-dependent uptake of D-glucose by pars convoluta and pars recta membrane vesicles could be described by single, but different, transport systems, namely a low-affinity system with KA congruent to 3.5 mM and a high-affinity system with KA congruent to 0.30 mM respectively. Attempts to calculate the stoichiometry of the different Na+/D-glucose transport systems by using Hill-type plots revealed that the ratio of the Na+/hexose co-transport probably is 1:1 in the case of pars convoluta and 2:1 in membrane vesicles from pars recta. The Na+/L-phenylalanine stoichiometry of the pars convoluta transporter probably is 1:1. Both the high-affinity and the low-affinity Na+-dependent L-phenylalanine transport system of pars recta membrane vesicles seem to operate with a 1:1 stoichiometry. The physiological importance of the arrangement of low-affinity and high-affinity transport systems along the kidney proximal tubule is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson P. S., Sacktor B. The Na+ gradient-dependent transport of D-glucose in renal brush border membranes. J Biol Chem. 1975 Aug 10;250(15):6032–6039. [PubMed] [Google Scholar]
  2. Aronson P. S., Sacktor B. Transport of D-glucose by brush border membranes isolated from the renal cortex. Biochim Biophys Acta. 1974 Jul 31;356(2):231–243. doi: 10.1016/0005-2736(74)90286-7. [DOI] [PubMed] [Google Scholar]
  3. Barfuss D. W., Schafer J. A. Active amino acid absorption by proximal convoluted and proximal straight tubules. Am J Physiol. 1979 Feb;236(2):F149–F162. doi: 10.1152/ajprenal.1979.236.2.F149. [DOI] [PubMed] [Google Scholar]
  4. Barfuss D. W., Schafer J. A. Differences in active and passive glucose transport along the proximal nephron. Am J Physiol. 1981 Sep;241(3):F322–F332. doi: 10.1152/ajprenal.1981.241.3.F322. [DOI] [PubMed] [Google Scholar]
  5. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  6. Evers J., Murer H., Kinne R. Phenylalanine uptake in isolated renal brush border vesicles. Biochim Biophys Acta. 1976 Apr 5;426(4):598–615. doi: 10.1016/0005-2736(76)90124-3. [DOI] [PubMed] [Google Scholar]
  7. Frömter E. Electrophysiological analysis of rat renal sugar and amino acid transport. I. Basic phenomena. Pflugers Arch. 1982 Apr;393(2):179–189. doi: 10.1007/BF00582942. [DOI] [PubMed] [Google Scholar]
  8. Jacobsen C., Frich J. R., Steensgaard J. Determination of affinity of monoclonal antibodies against human IgG. J Immunol Methods. 1982;50(1):77–88. doi: 10.1016/0022-1759(82)90305-2. [DOI] [PubMed] [Google Scholar]
  9. Jørgensen K. E., Kragh-Hansen U., Røigaard-Petersen H., Sheikh M. I. Citrate uptake by basolateral and luminal membrane vesicles from rabbit kidney cortex. Am J Physiol. 1983 Jun;244(6):F686–F695. doi: 10.1152/ajprenal.1983.244.6.F686. [DOI] [PubMed] [Google Scholar]
  10. Kaissling B., Kriz W. Structural analysis of the rabbit kidney. Adv Anat Embryol Cell Biol. 1979;56:1–123. doi: 10.1007/978-3-642-67147-0. [DOI] [PubMed] [Google Scholar]
  11. Kragh-Hansen U., Jørgensen K. E., Sheikh M. I. The use of a potential-sensitive cyanine dye for studying ion-dependent electrogenic renal transport of organic solutes. Uptake of L-malate and D-malate by luminal-membrane vesicles. Biochem J. 1982 Nov 15;208(2):369–376. doi: 10.1042/bj2080369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kragh-Hansen U., Jørgensen K. E., Sheikh M. I. The use of potential-sensitive cyanine dye for studying ion-dependent electrogenic renal transport of organic solutes. Spectrophotometric measurements. Biochem J. 1982 Nov 15;208(2):359–368. doi: 10.1042/bj2080359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Ling K. Y., Im W. B., Faust R. G. Na+-independent sugar uptake by rat intestinal and renal brush border and basolateral membrane vesicles. Int J Biochem. 1981;13(6):693–700. doi: 10.1016/0020-711x(81)90037-9. [DOI] [PubMed] [Google Scholar]
  15. Nord E., Wright S. H., Kippen I., Wright E. M. Pathways for carboxylic acid transport by rabbit renal brush border membrane vesicles. Am J Physiol. 1982 Nov;243(5):F456–F462. doi: 10.1152/ajprenal.1982.243.5.F456. [DOI] [PubMed] [Google Scholar]
  16. Ottolenghi P. The reversible delipidation of a solubilized sodium-plus-potassium ion-dependent adenosine triphosphatase from the salt gland of the spiny dogfish. Biochem J. 1975 Oct;151(1):61–66. doi: 10.1042/bj1510061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  18. Rostgaard J., Møller O. Localization of Na+, K+ -ATPase to the inside of the basolateral cell membranes of epithelial cells of proximal and distal tubules in rabbit kidney. Cell Tissue Res. 1980;212(1):17–28. doi: 10.1007/BF00234029. [DOI] [PubMed] [Google Scholar]
  19. Samarzija I., Frömter E. Electrophysiological analysis of rat renal sugar and amino acid transport. III. Neutral amino acids. Pflugers Arch. 1982 May;393(3):119–209. [PubMed] [Google Scholar]
  20. Samarzija I., Hinton B. T., Frömter E. Electrophysiological analysis of rat renal sugar and amino acid transport. II. Dependence on various transport parameters and inhibitors. Pflugers Arch. 1982 Apr;393(2):190–197. doi: 10.1007/BF00582943. [DOI] [PubMed] [Google Scholar]
  21. Sheikh M. I., Kragh-Hansen U., Jørgensen K. E., Røigaard-Petersen H. An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex. Biochem J. 1982 Nov 15;208(2):377–382. doi: 10.1042/bj2080377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sheikh M. I. Renal handling of phenol red. I. A comparative study on the accumulation of phenol red and p-aminohippurate in rabbit kidney tubules in vitro. J Physiol. 1972 Dec;227(2):565–590. doi: 10.1113/jphysiol.1972.sp010048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Silbernagl S. Physiologie und Pathophysiologie der renalen Aminosäuren-Resorption. Padiatr Padol. 1981;16(1):9–22. [PubMed] [Google Scholar]
  24. Turner R. J., Moran A. Further studies of proximal tubular brush border membrane D-glucose transport heterogeneity. J Membr Biol. 1982;70(1):37–45. doi: 10.1007/BF01871587. [DOI] [PubMed] [Google Scholar]
  25. Turner R. J., Moran A. Heterogeneity of sodium-dependent D-glucose transport sites along the proximal tubule: evidence from vesicle studies. Am J Physiol. 1982 Apr;242(4):F406–F414. doi: 10.1152/ajprenal.1982.242.4.F406. [DOI] [PubMed] [Google Scholar]
  26. Turner R. J., Moran A. Stoichiometric studies of the renal outer cortical brush border membrane D-glucose transporter. J Membr Biol. 1982;67(1):73–80. doi: 10.1007/BF01868649. [DOI] [PubMed] [Google Scholar]

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