Abstract
Cultured human lung fibroblasts, incubated with cholesterol/phosphatidylcholine vesicles (cholesterol: phosphatidylcholine molar ratio 1:1) incorporated vesicle [3H]-cholesterol linearly for at least 48 h by an exchange process without gaining sterol mass. The incorporation of [3H]cholesterol by the cells was markedly enhanced in the presence of purified bovine serum albumin. A fraction of the incorporated vesicle [3H]cholesterol was esterified by the cells.
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- Gamble W., Vaughan M., Kruth H. S., Avigan J. Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells. J Lipid Res. 1978 Nov;19(8):1068–1070. [PubMed] [Google Scholar]
- Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
- Mayhew E., Gotfredsen C., Schneider Y. J., Trouet A. Interaction of liposomes with cultured cells: effect of serum. Biochem Pharmacol. 1980 Mar 15;29(6):877–886. doi: 10.1016/0006-2952(80)90217-8. [DOI] [PubMed] [Google Scholar]
- McLean L. R., Phillips M. C. Mechanism of cholesterol and phosphatidylcholine exchange or transfer between unilamellar vesicles. Biochemistry. 1981 May 12;20(10):2893–2900. doi: 10.1021/bi00513a028. [DOI] [PubMed] [Google Scholar]
- Poznansky M. J., Czekanski S. Cholesterol movement between human skin fibroblasts and phosphatidylcholine vesicles. Biochim Biophys Acta. 1982 Feb 23;685(2):182–190. doi: 10.1016/0005-2736(82)90096-7. [DOI] [PubMed] [Google Scholar]
- Riordan J. R., Alon N., Buchwald M. Plasma membrane lipids of human diploid fibroblasts from normal individuals and patients with cystic fibrosis. Biochim Biophys Acta. 1979 Jul 27;574(1):39–47. doi: 10.1016/0005-2760(79)90082-1. [DOI] [PubMed] [Google Scholar]
- Rothblat G. H., Arbogast L. Y., Ouellette L., Howard B. V. Preparation of delipidized serum protein for use in cell culture systems. In Vitro. 1976 Aug;12(8):554–557. doi: 10.1007/BF02797438. [DOI] [PubMed] [Google Scholar]
- Rothblat G. H., Phillips M. C. Mechanism of cholesterol efflux from cells. Effects of acceptor structure and concentration. J Biol Chem. 1982 May 10;257(9):4775–4782. [PubMed] [Google Scholar]
- Rottem S., Shinar D., Bittman R. Symmetrical distribution and rapid transbilayer movement of cholesterol in Mycoplasma gallisepticum membranes. Biochim Biophys Acta. 1981 Dec 21;649(3):572–580. doi: 10.1016/0005-2736(81)90161-9. [DOI] [PubMed] [Google Scholar]
- Slotte J. P., Lundberg B. Transfer of [3H]cholesterol between lipid vesicles and rat arterial smooth muscle cells in vitro. Biochim Biophys Acta. 1983 Mar 1;750(3):434–439. doi: 10.1016/0005-2760(83)90182-0. [DOI] [PubMed] [Google Scholar]
- Tyrrell D. A., Richardson V. J., Ryman B. E. The effect of serum protein fractions on liposome-cell interactions in cultured cells and the perfused rat liver. Biochim Biophys Acta. 1977 Apr 27;497(2):469–480. doi: 10.1016/0304-4165(77)90204-5. [DOI] [PubMed] [Google Scholar]
- Wharton S. A., Green C. Effect of sterol structure on the transfer of sterols and phospholipids from liposomes to erythrocytes in vitro. Biochim Biophys Acta. 1982 Jun 11;711(3):398–402. doi: 10.1016/0005-2760(82)90053-4. [DOI] [PubMed] [Google Scholar]