Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Jun 15;220(3):767–772. doi: 10.1042/bj2200767

The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase.

F X Rius, P F Knowles, G Pettersson
PMCID: PMC1153694  PMID: 6466302

Abstract

The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies were made under both air and O2-saturating conditions. The results establish unequivocally that NH3 is released in the step whereby a reduced enzyme species is re-oxidized by molecular O2 rather than in the step leading to the reduced enzyme. It is concluded that the catalytic cycle of the enzyme conforms to an aminotransferase mechanism rather than one in which an imine is an intermediate.

Full text

PDF
767

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLASCHKO H., BUFFONI F. PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:45–60. doi: 10.1098/rspb.1965.0059. [DOI] [PubMed] [Google Scholar]
  2. Barker R., Boden N., Cayley G., Charlton S. C., Henson R., Holmes M. C., Kelly I. D., Knowles P. F. Properties of cupric ions in benzylamine oxidase from pig plasma as studied by magnetic-resonance and kinetic methods. Biochem J. 1979 Jan 1;177(1):289–302. doi: 10.1042/bj1770289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg K. A., Abeles R. H. Mechanism of action of plasma amine oxidase products released under anaerobic conditions. Biochemistry. 1980 Jul 8;19(14):3186–3189. doi: 10.1021/bi00555a012. [DOI] [PubMed] [Google Scholar]
  4. Grant J., Kelly I., Knowles P., Olsson J., Pettersson G. Changes in the copper centres of benzylamine oxidase from pig plasma during the catalytic cycle. Biochem Biophys Res Commun. 1978 Aug 14;83(3):1216–1224. doi: 10.1016/0006-291x(78)91524-3. [DOI] [PubMed] [Google Scholar]
  5. Kalb V. F., Jr, Donohue T. J., Corrigan M. G., Bernlohr R. W. A new and specific assay for ammonia and glutamine sensitive to 100 pmol. Anal Biochem. 1978 Oct 1;90(1):47–57. doi: 10.1016/0003-2697(78)90007-6. [DOI] [PubMed] [Google Scholar]
  6. Lindström A., Olsson B., Olsson J., Pettersson G. Effect of pH on the transient reduction of pig-plasma benzylamine oxidase by benzylamine derivatives. Eur J Biochem. 1976 May 1;64(2):321–326. doi: 10.1111/j.1432-1033.1976.tb10304.x. [DOI] [PubMed] [Google Scholar]
  7. Lindström A., Pettersson G. Active-site titration of pig-plasma benzylamine oxidase. Eur J Biochem. 1978 Feb 1;83(1):131–135. doi: 10.1111/j.1432-1033.1978.tb12076.x. [DOI] [PubMed] [Google Scholar]
  8. Lindström A., Pettersson G. The order of substrate addition and product release during the catalytic action of pig-plasma benzylamine oxidase. Eur J Biochem. 1978 Mar 15;84(2):479–485. doi: 10.1111/j.1432-1033.1978.tb12189.x. [DOI] [PubMed] [Google Scholar]
  9. Olsson B., Olsson J., Pettersson G. Effects on enzyme activity of ligand-binding to copper in pig-plasma benzylamine oxidase. Eur J Biochem. 1978 Jun 1;87(1):1–8. doi: 10.1111/j.1432-1033.1978.tb12345.x. [DOI] [PubMed] [Google Scholar]
  10. Olsson B., Olsson J., Pettersson G. The kinetics of reoxidation of reduced benzylamine oxidase. Eur J Biochem. 1977 Apr 1;74(2):329–335. doi: 10.1111/j.1432-1033.1977.tb11397.x. [DOI] [PubMed] [Google Scholar]
  11. Pettersson G. A generalized theoretical treatment of the transient-state kinetics of enzymic reaction systems far from equilibrium. Acta Chem Scand B. 1978;32(6):437–446. doi: 10.3891/acta.chem.scand.32b-0437. [DOI] [PubMed] [Google Scholar]
  12. Suva R. H., Abeles R. H. Studies on the mechanism of action of plasma amine oxidase. Biochemistry. 1978 Aug 22;17(17):3538–3545. doi: 10.1021/bi00610a018. [DOI] [PubMed] [Google Scholar]
  13. TABOR C. W., TABOR H., ROSENTHAL S. M. Purification of amine oxidase from beef plasma. J Biol Chem. 1954 Jun;208(2):645–661. [PubMed] [Google Scholar]
  14. Taylor C. E., Taylor R. S., Rasmussen C., Knowles P. F. A catalytic mechanism for the enzyme benzylamine oxidase from pig plasma. Biochem J. 1972 Dec;130(3):713–728. doi: 10.1042/bj1300713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yadav K. D., Knowles P. F. A catalytic mechanism for benzylamine oxidase from pig plasma. Stopped-flow kinetic studies. Eur J Biochem. 1981;114(1):139–144. doi: 10.1111/j.1432-1033.1981.tb06184.x. [DOI] [PubMed] [Google Scholar]
  16. Yasunobu K. T., Ishizaki H., Minamiura N. The molecular mechanistic and immunological properties of amine oxidases. Mol Cell Biochem. 1976 Oct 30;13(1):3–29. doi: 10.1007/BF01732392. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES