Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1984 Jun 15;220(3):849–852. doi: 10.1042/bj2200849

The identification of threonine-95 as the major site of glycosylation in normal human myelin basic protein.

T F Cruz, D D Wood, M A Moscarello
PMCID: PMC1153705  PMID: 6205652

Abstract

The enzymic transfer of N-acetylgalactosamine to myelin basic protein and that to peptides derived from basic protein were compared. Basic protein treated with pepsin and trypsin before glycosylation resulted in decreases of 7% and 23% in the amount of N-acetylgalactosamine transferred to the peptides respectively. However, digestion of basic protein had little effect on the sites glycosylated. It was found that Thr-95 was the major site for glycosylation in both the intact human basic protein and in the tryptic peptides.

Full text

PDF
849

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boggs J. M., Wood D. D., Moscarello M. A. Hydrophobic and electrostatic interactions of myelin basic proteins with lipid. Participation of N-terminal and C-terminal portions. Biochemistry. 1981 Mar 3;20(5):1065–1073. doi: 10.1021/bi00508a004. [DOI] [PubMed] [Google Scholar]
  2. Briand J. P., Andrews S. P., Jr, Cahill E., Conway N. A., Young J. D. Investigation of the requirements for O-glycosylation by bovine submaxillary gland UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosamine transferase using synthetic peptide substrates. J Biol Chem. 1981 Dec 10;256(23):12205–12207. [PubMed] [Google Scholar]
  3. CARLSON D. M., SWANSON A. L., ROSEMAN S. PREPARATION OF CRYSTALLINE ALPHA-D-GALACTOSAMINE-1-PHOSPHORIC ACID AND ITS CONVERSION TO UDP-N-ACETYLGALACTOSAMINE. Biochemistry. 1964 Mar;3:402–405. doi: 10.1021/bi00891a016. [DOI] [PubMed] [Google Scholar]
  4. Cruz T. F., Moscarello M. A. Identification of the major sites of enzymic glycosylation of myelin basic protein. Biochim Biophys Acta. 1983 Nov 8;760(3):403–410. doi: 10.1016/0304-4165(83)90381-1. [DOI] [PubMed] [Google Scholar]
  5. Hagopian A., Westall F. C., Whitehead J. S., Eylar E. H. Glycosylation of the A1 protein from myelin by a polypeptide N-acetylgalactosaminyltransferase. Identification of the receptor sequence. J Biol Chem. 1971 Apr 25;246(8):2519–2523. [PubMed] [Google Scholar]
  6. Lowden J. A., Moscarello M. A., Morecki R. The isolation and characterization of an acid-soluble protein from myelin. Can J Biochem. 1966 May;44(5):567–577. doi: 10.1139/o66-068. [DOI] [PubMed] [Google Scholar]
  7. Mendz G. L., Moore W. J., Martenson R. E. NMR studies on myelin basic protein. VIII. Complete assignment of the threonine residues by proton NMR of proteins from five species. Biochim Biophys Acta. 1983 Jan 12;742(1):215–223. doi: 10.1016/0167-4838(83)90379-5. [DOI] [PubMed] [Google Scholar]
  8. Pless D. D., Lennarz W. J. Enzymatic conversion of proteins to glycoproteins. Proc Natl Acad Sci U S A. 1977 Jan;74(1):134–138. doi: 10.1073/pnas.74.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schwyzer M., Hill R. L. Porcine A blood group-specific N-acetylgalactosaminyltransferase. I. Purification from porcine submaxillary glands. J Biol Chem. 1977 Apr 10;252(7):2338–2345. [PubMed] [Google Scholar]
  10. Sugiura M., Kawasaki T., Yamashina I. Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosamine transferase from an ascites hepatoma, AH 66. J Biol Chem. 1982 Aug 25;257(16):9501–9507. [PubMed] [Google Scholar]
  11. Young J. D., Tsuchiya D., Sandlin D. E., Holroyde M. J. Enzymic O-glycosylation of synthetic peptides from sequences in basic myelin protein. Biochemistry. 1979 Oct 2;18(20):4444–4448. doi: 10.1021/bi00587a026. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES