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. 1982 Nov 15;208(2):425–433. doi: 10.1042/bj2080425

Directional Ca2+ effect on stimulation of mucin secretion from chicken trachea in vitro

Nasi Mian *, Caroline E Anderson *, Andrew J Pope *, Anthony R Smith *, Paul S Richardson †, Kim Balfre †, Paul W Kent *
PMCID: PMC1153980  PMID: 7159411

Abstract

Chicken tracheal mucosa in vitro transported and incorporated radioactive precursors into mucins, which were secreted at a steady rate into the tracheal lumen. Secretion of mucins labelled with 35S and 3H after pulse-labelling of the mucosal layer with Na235SO4 and d-[1-3H]glucosamine as precursors was an energy-dependent process, as it was strongly inhibited by the action of respiratory-chain inhibitors, an uncoupler of oxidative phosphorylation, a metabolic blocker and a temperature shift from 41°C to 5°C. On the other hand, both cholinergic and parasympathomimetic agents considerably increased the secretion of dual-radiolabelled mucins when applied on the submucosal side of the trachea. The effect of Ca2+ was directional, since only high submucosal (3.6 or 18mm) or low luminal (zero or 0.18mm) Ca2+ massively enhanced the secretion of radiolabelled mucin compared with the mucin output measured under physiological Ca2+ conditions (1.8mm). Whereas application of ionophore A23187 on either side of the trachea significantly increased mucin output, its presence in the appropriate tracheal compartment and under appropriate Ca2+ conditions further accentuated the output of radiolabelled mucins. Addition of acetylcholine under appropriate conditions also had an additive effect on the Ca2+-stimulated secretion of mucins. Ca2+ stimulation of mucin secretion appears to be dependent on the metabolic integrity of the mucosal cells. Mucins secreted in response to high submucosal and low luminal [Ca2+] appear to consist of a number of different types of glycoproteins, as judged from their ion-exchange-chromatographic behaviour.

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Selected References

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  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. Carafoli E. Mitochondrial uptake of calcium ions and the regulation of cell function. Biochem Soc Symp. 1974;(39):89–109. [PubMed] [Google Scholar]
  3. Clamp J. R., Bhatti T., Chambers R. E. The determination of carbohydrate in biological materials by gas-liquid chromatography. Methods Biochem Anal. 1971;19:229–344. doi: 10.1002/9780470110386.ch3. [DOI] [PubMed] [Google Scholar]
  4. Dahl G., Ekerdt R., Gratzl M. Models for exocytotic membrane fusion. Symp Soc Exp Biol. 1979;33:349–368. [PubMed] [Google Scholar]
  5. Douglas W. W. Involvement of calcium in exocytosis and the exocytosis--vesiculation sequence. Biochem Soc Symp. 1974;(39):1–28. [PubMed] [Google Scholar]
  6. Douglas W. W. Stimulus-secretion coupling: the concept and clues from chromaffin and other cells. Br J Pharmacol. 1968 Nov;34(3):451–474. doi: 10.1111/j.1476-5381.1968.tb08474.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kent P. W. Chemical aspects of tracheal glycoproteins. Ciba Found Symp. 1978;(54):155–174. doi: 10.1002/9780470720356.ch8. [DOI] [PubMed] [Google Scholar]
  8. Mantle M., Allen A. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain [proceedings]. Biochem Soc Trans. 1978;6(3):607–609. doi: 10.1042/bst0060607. [DOI] [PubMed] [Google Scholar]
  9. Mela L. Interactions of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake. Arch Biochem Biophys. 1968 Feb;123(2):286–293. doi: 10.1016/0003-9861(68)90136-7. [DOI] [PubMed] [Google Scholar]
  10. Mende T. J., Whitney P. L. Microdetermination of inorganic sulfate using thin-layer plates. Anal Biochem. 1978 Feb;84(2):570–573. doi: 10.1016/0003-2697(78)90076-3. [DOI] [PubMed] [Google Scholar]
  11. Mian N., Anderson C. E., Pope A. J., Kent P. W. Physicochemical properties of avian tracheal mucus. Biochem J. 1982 Mar 1;201(3):533–542. doi: 10.1042/bj2010533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mian N., Pope A. J., Anderson C. E., Kent P. W. Factors influencing the viscous properties of chicken tracheal mucins. Biochim Biophys Acta. 1982 Jul 16;717(1):41–48. doi: 10.1016/0304-4165(82)90377-4. [DOI] [PubMed] [Google Scholar]
  13. Rahwan R. G., Borowitz J. L., Miya T. S. The role of intracellular calcium in catecholamine secretion from the bovine adrenal medulla. J Pharmacol Exp Ther. 1973 Jan;184(1):106–118. [PubMed] [Google Scholar]
  14. Richardson P. S., Phipps R. J., Balfre K., Hall R. L. The roles of mediators, irritants and allergens in causing mucin secretion from the trachea. Ciba Found Symp. 1978;(54):111–131. doi: 10.1002/9780470720356.ch6. [DOI] [PubMed] [Google Scholar]
  15. Rubin R. P. The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol Rev. 1970 Sep;22(3):389–428. [PubMed] [Google Scholar]
  16. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]

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