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. Author manuscript; available in PMC: 2024 Nov 6.
Published in final edited form as: Biochemistry. 2019 Dec 2;59(4):389–399. doi: 10.1021/acs.biochem.9b00771

Table 1.

Measurement of the Binding Affinities of TCF19-PHD WT and Its Mutants with H3K4me3 Peptides As Obtained from NMR

protein peptide Kd
TCF19 (DDCAC) PHD H3K4me3 4.7 ± 0.4 μMa
TCF19 PHD V305Y H3K4me3 weak binding
TCF19 PHD A306Y unstable
TCF19 PHD Q309R unstable
TCF19 PHD D311R unstable
TCF19 PHD D314R unstable
TCF19 PHD C324A H3K4me3 440 ± 170 μM
TCF19 PHD R330Y H3K4me3 400 ± 60 μM
TCF19 PHD W307A unfolded
TCF19 PHD W307Y H3K4me3 2.0 ± 0.6 mM
TCF19 PHD W316A unfolded
TCF19 PHD W316Y H3K4me3 no binding
a

Obtained from tryptophan fluorescence spectroscopy.