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. 1983 Jan 1;209(1):257–260. doi: 10.1042/bj2090257

A simple assay to study protein-mediated lipid exchange by fluorescence polarization.

Y H Xü, K Gietzen, H J Galla, E Sackmann
PMCID: PMC1154080  PMID: 6847612

Abstract

We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid phase transitions. From the heights of each phase-transition step we were able to follow the lipid transfer process without separating the two vesicle preparations.

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Selected References

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