Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Mar 1;209(3):627–633. doi: 10.1042/bj2090627

Localization of beta-glucan synthases on the membranes of cultured Lolium multiflorum (ryegrass) endosperm cells.

R J Henry, A Schibeci, B A Stone
PMCID: PMC1154139  PMID: 6223621

Abstract

The distribution of beta-glucan synthases between plasma membranes and intracellular membranes of suspension-cultured Italian-ryegrass (Lolium multiflorum Lam.) endosperm cells was examined. Highly purified plasma membranes prepared from protoplasts were only slightly enriched in beta-glucan synthases assayed at 10 microM- and 1 mM-UDP-glucose. Most beta-glucan synthase was associated with intracellular membranes. These membranes were fractionated on a linear sucrose density gradient and were resolved into different membrane fractions containing beta-glucan synthases. Beta-Glucan synthases assayed at 10 microM-UDP-glucose were found in a fraction banding at a density of 1.11 g . cm-3, but most of the beta-glucan synthase assayed at 1 mM-DDP-glucose was at a density of 1.04 g . cm-3.

Full text

PDF
627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. L., Ray P. M. Labeling of the Plasma Membrane of Pea Cells by a Surface-localized Glucan Synthetase. Plant Physiol. 1978 May;61(5):723–730. doi: 10.1104/pp.61.5.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boller T., Kende H. Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol. 1979 Jun;63(6):1123–1132. doi: 10.1104/pp.63.6.1123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chadwick C. M., Northcote D. H. Glucosylation of phosphorylpolyisoprenol and sterol at the plasma membrane of soya-bean (Glycine max) protoplasts. Biochem J. 1980 Feb 15;186(2):411–421. doi: 10.1042/bj1860411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Henry R. J., Stone B. A. Factors Influencing beta-Glucan Synthesis by Particulate Enzymes from Suspension-Cultured Lolium multiflorum Endosperm Cells. Plant Physiol. 1982 Mar;69(3):632–636. doi: 10.1104/pp.69.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Heslop-Harrison J., Heslop-Harrison Y. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain Technol. 1970 May;45(3):115–120. doi: 10.3109/10520297009085351. [DOI] [PubMed] [Google Scholar]
  6. Hodges T. K., Leonard R. T. Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol. 1974;32:392–406. doi: 10.1016/0076-6879(74)32039-3. [DOI] [PubMed] [Google Scholar]
  7. Pearce R. S., Withers L. A., Willison J. H. Bodies of wall-like material ("wall-bodies") produced intracellularly by cultured isolated protoplasts and plasmolysed cells of higher plants. Protoplasma. 1974;82(3):223–236. doi: 10.1007/BF01276309. [DOI] [PubMed] [Google Scholar]
  8. Ray P. M., Shininger T. L., Ray M. M. ISOLATION OF beta-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANES. Proc Natl Acad Sci U S A. 1969 Oct;64(2):605–612. doi: 10.1073/pnas.64.2.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rothman J. E. The golgi apparatus: two organelles in tandem. Science. 1981 Sep 11;213(4513):1212–1219. doi: 10.1126/science.7268428. [DOI] [PubMed] [Google Scholar]
  10. Schibeci A., Fincher G. B., Stone B. A., Wardrop A. B. Isolation of plasma membrane from protoplasts of Lolium multiflorum (ryegrass) endosperm cells. Biochem J. 1982 Sep 1;205(3):511–519. doi: 10.1042/bj2050511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith M. M., Stone B. A. Beta-glucan synthesis by cell-free extracts from Lolium multiflorum endosperm. Biochim Biophys Acta. 1973 Jun 20;313(1):72–94. doi: 10.1016/0304-4165(73)90189-x. [DOI] [PubMed] [Google Scholar]
  12. TAUSSKY H. H., SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]
  13. Van Der Woude W. J., Lembi C. A., Morré D. J. beta-Glucan Synthetases of Plasma Membrane and Golgi Apparatus from Onion Stem. Plant Physiol. 1974 Sep;54(3):333–340. doi: 10.1104/pp.54.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES