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. 1983 Feb 15;210(2):509–515. doi: 10.1042/bj2100509

Induction by growth factors of polysaccharide synthases in bean cell suspension cultures.

G P Bolwell, D H Northcote
PMCID: PMC1154251  PMID: 6860309

Abstract

Suspension cells of bean subcultured into medium that maintains the culture and stimulates cell division but not differentiation brings about an increase in arabinan synthase activity. Subculture into a medium that induces both cell division and xylogenesis brings about in addition an increase in xylan synthase. Both synthases are membrane-bound and are concerned with the formation of neutral pectin or hemicellulose of the cell wall respectively. During the rising phase of the induction of these activities in the appropriate culture medium, the increases in activities were inhibited by either actinomycin D (an inhibitor of transcription) or D-2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide (an inhibitor of translation). Thus the control for the induction of the enzyme activities involves transcription and possibly translation. Subculture of the cells brought about an increase, probably non-specific, in total membrane-bound translation, as indicated by increased amounts of bound polysomes and incorporation of [35S]methionine into membrane proteins. If the control of the appearance of specific mRNA molecules is partially effected by growth factors then these are probably operative during the period of the cell cycle that is stimulated by subculture and it is probably at this time that the growth factors act to bring about the changes necessary for differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevan M., Northcote D. H. The loss of morphogenetic potential and induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris. J Cell Sci. 1979 Oct;39:339–353. doi: 10.1242/jcs.39.1.339. [DOI] [PubMed] [Google Scholar]
  2. Bolwell G. P., Northcote D. H. Arabinan synthase and xylan synthase activities of Phaseolus vulgaris. Subcellular localization and possible mechanism of action. Biochem J. 1983 Feb 15;210(2):497–507. doi: 10.1042/bj2100497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brett C. T., Northcote D. H. The formation of oligoglucans linked to lipid during synthesis of beta-glucan by characterized membrane fractions isolated from peas. Biochem J. 1975 Apr;148(1):107–117. doi: 10.1042/bj1480107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dalessandro G., Northcote D. H. Changes in enzymic activities of nucleoside diphosphate sugar interconversions during differentiation of cambium to xylem in pine and fir. Biochem J. 1977 Feb 15;162(2):281–288. doi: 10.1042/bj1620281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dalessandro G., Northcote D. H. Changes in enzymic activities of nucleoside diphosphate sugar interconversions during differentiation of cambium to xylem in sycamore and poplar. Biochem J. 1977 Feb 15;162(2):267–279. doi: 10.1042/bj1620267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  7. Goldberg I. H., Friedman P. A. Antibiotics and nucleic acids. Annu Rev Biochem. 1971;40:775–810. doi: 10.1146/annurev.bi.40.070171.004015. [DOI] [PubMed] [Google Scholar]
  8. Haddon L. E., Northcote D. H. Quantitative measurement of the course of bean callus differentiation. J Cell Sci. 1975 Jan;17(1):11–26. doi: 10.1242/jcs.17.1.11. [DOI] [PubMed] [Google Scholar]
  9. Jones D. H., Northcote D. H. Induction by hormones of phenylalanine ammonia-lyase in bean-cell suspension cultures. Inhibition of superinduction by actinomycin D. Eur J Biochem. 1981 May;116(1):117–125. doi: 10.1111/j.1432-1033.1981.tb05308.x. [DOI] [PubMed] [Google Scholar]
  10. Ray P. M. Cooperative action of beta-glucan synthetase and UDP-xylose xylosyl transferase of Golgi membranes in the synthesis of xyloglucan-like polysaccharide. Biochim Biophys Acta. 1980 May 22;629(3):431–444. doi: 10.1016/0304-4165(80)90149-x. [DOI] [PubMed] [Google Scholar]
  11. Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
  12. Roberts K., Northcote D. H. The structure of sycamore callus cells during division in a partially synchronized suspension culture. J Cell Sci. 1970 Mar;6(2):299–321. doi: 10.1242/jcs.6.2.299. [DOI] [PubMed] [Google Scholar]
  13. Tomkins G. M., Levinson B. B., Baxter J. D., Dethlefsen L. Further evidence for posttranscriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells. Nat New Biol. 1972 Sep 6;239(88):9–14. doi: 10.1038/newbio239009a0. [DOI] [PubMed] [Google Scholar]
  14. Weeks D. P., Baxter R. Specific inhibition of peptide-chain initiation by 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide. Biochemistry. 1972 Aug 1;11(16):3060–3064. doi: 10.1021/bi00766a018. [DOI] [PubMed] [Google Scholar]

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