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. 1983 Mar 15;210(3):867–873. doi: 10.1042/bj2100867

alpha-adrenergic stimulation of respiration in isolated rat hepatocytes.

A Binet, M Claret
PMCID: PMC1154301  PMID: 6409081

Abstract

1. The alpha-adrenergic agonists noradrenaline (in the presence of beta-blocker) and phenylephrine cause a transient stimulation of the respiration in isolated rat hepatocytes. After a lag period of 12s, this activation first attains its maximal value (+24%) for about 1 min and then falls to a sustained value (+15%). The effect is blocked by the alpha-antagonists phenoxybenzamine and phentolamine. It is dose-dependent, with an half-maximal stimulation by 16 nM-noradrenaline, which is similar to that found for other cell responses to the hormone. 2. Vasopressin and ATP, which in common with alpha-agonists are believed to increase intracellular [Ca2+], induce similar activation in the respiration rate. 3. The alpha-adrenergic-mediated respiration depends on extracellular Ca2+. The activation is decreased or abolished when extracellular [Ca2+] is decreased by adding EGTA, or when the Ca2+ antagonists Mn2+ and La3+ are present in the incubation medium. 4. It is suggested that the activation of the mitochondrial respiration rate results from the increase in cytosolic Ca2+ concentration, presumably via Ca2+ influx or Ca2+ release from the plasma membrane or endoplasmic reticulum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Althaus-Salzmann M., Carafoli E., Jakob A. Ca2+, K+ redistributions and alpha-adrenergic activation of glycogenolysis in perfused rat livers. Eur J Biochem. 1980 May;106(1):241–248. doi: 10.1111/j.1432-1033.1980.tb06015.x. [DOI] [PubMed] [Google Scholar]
  2. Bartfai T. Preparation of metal-chelate complexes and the design of steady-state kinetic experiments involving metal nucleotide complexes. Adv Cyclic Nucleotide Res. 1979;10:219–242. [PubMed] [Google Scholar]
  3. Berthon B., Poggioli J., Capiod T., Claret M. Effect of the alpha-agonist noradrenaline on total and 45Ca2+ movements in mitochondria of rat liver cells. Biochem J. 1981 Oct 15;200(1):177–180. doi: 10.1042/bj2000177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blackmore P. F., Brumley F. T., Marks J. L., Exton J. H. Studies on alpha-adrenergic activation of hepatic glucose output. Relationship between alpha-adrenergic stimulation of calcium efflux and activation of phosphorylase in isolated rat liver parenchymal cells. J Biol Chem. 1978 Jul 25;253(14):4851–4858. [PubMed] [Google Scholar]
  5. Blackmore P. F., Dehaye J. P., Exton J. H. Studies on alpha-adrenergic activation of hepatic glucose output. The role of mitochondrial calcium release in alpha-adrenergic activation of phosphorylase in perfused rat liver. J Biol Chem. 1979 Aug 10;254(15):6945–6950. [PubMed] [Google Scholar]
  6. Blackmore P. F., Hughes B. P., Shuman E. A., Exton J. H. alpha-Adrenergic activation of phosphorylase in liver cells involves mobilization of intracellular calcium without influx of extracellular calcium. J Biol Chem. 1982 Jan 10;257(1):190–197. [PubMed] [Google Scholar]
  7. Burgess G. M., Claret M., Jenkinson D. H. Effects of catecholamines, ATP and ionophore A23187 on potassium and calcium movements in isolated hepatocytes. Nature. 1979 Jun 7;279(5713):544–546. doi: 10.1038/279544a0. [DOI] [PubMed] [Google Scholar]
  8. Burgess G. M., Claret M., Jenkinson D. H. Effects of quinine and apamin on the calcium-dependent potassium permeability of mammalian hepatocytes and red cells. J Physiol. 1981 Aug;317:67–90. doi: 10.1113/jphysiol.1981.sp013814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Capiod T., Berthon B., Poggioli J., Burgess G. M., Claret M. The effect of Ca2+ -mobilising hormones on the Na+ --K+ pump in isolated rat liver hepatocytes. FEBS Lett. 1982 May 3;141(1):49–52. doi: 10.1016/0014-5793(82)80013-6. [DOI] [PubMed] [Google Scholar]
  10. Chen J. L., Babcock D. F., Lardy H. A. Norepinephrine, vasopressin, glucagon, and A23187 induce efflux of calcium from an exchangeable pool in isolated rat hepatocytes. Proc Natl Acad Sci U S A. 1978 May;75(5):2234–2238. doi: 10.1073/pnas.75.5.2234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Claret-Berthon B., Claret M., Mazet J. L. Fluxes and distribution of calcium in rat liver cells: kinetic analysis and identification of pools. J Physiol. 1977 Nov;272(3):529–552. doi: 10.1113/jphysiol.1977.sp012058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Coll K. E., Joseph S. K., Corkey B. E., Williamson J. R. Determination of the matrix free Ca2+ concentration and kinetics of Ca2+ efflux in liver and heart mitochondria. J Biol Chem. 1982 Aug 10;257(15):8696–8704. [PubMed] [Google Scholar]
  13. Dehaye J. P., Hughes B. P., Blackmore P. F., Exton J. H. Insulin inhibition of alpha-adrenergic actions in liver. Biochem J. 1981 Mar 15;194(3):949–956. doi: 10.1042/bj1940949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Denton R. M., McCormack J. G. On the role of the calcium transport cycle in heart and other mammalian mitochondria. FEBS Lett. 1980 Sep 22;119(1):1–8. doi: 10.1016/0014-5793(80)80986-0. [DOI] [PubMed] [Google Scholar]
  15. Deth R., Lynch C. Inhibition of alpha-receptor-induced Ca2+ release and Ca2+ influx by Mn2+ and La3+. Eur J Pharmacol. 1981 Apr 24;71(1):1–11. doi: 10.1016/0014-2999(81)90381-2. [DOI] [PubMed] [Google Scholar]
  16. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  17. El-Refai M. F., Blackmore P. F., Exton J. H. Evidence for two alpha-adrenergic binding sites in liver plasma membranes. Studies with [3H]epinephrine and [3H]dihydroergocryptine. J Biol Chem. 1979 Jun 10;254(11):4375–4386. [PubMed] [Google Scholar]
  18. Exton J. H. Molecular mechanisms involved in alpha-adrenergic responses. Mol Cell Endocrinol. 1981 Sep;23(3):233–264. doi: 10.1016/0303-7207(81)90123-4. [DOI] [PubMed] [Google Scholar]
  19. Garrison J. C., Borland M. K., Florio V. A., Twible D. A. The role of calcium ion as a mediator of the effects of angiotensin II, catecholamines, and vasopressin on the phosphorylation and activity of enzymes in isolated hepatocytes. J Biol Chem. 1979 Aug 10;254(15):7147–7156. [PubMed] [Google Scholar]
  20. Green R. D., Dale M. M., Haylett D. G. Effect of adrenergic amines on the membrane potential of guinea-pig liver parenchymal cells in short term tissue culture. Experientia. 1972 Sep 15;28(9):1073–1074. doi: 10.1007/BF01918681. [DOI] [PubMed] [Google Scholar]
  21. Hansford R. G. Effect of micromolar concentrations of free Ca2+ ions on pyruvate dehydrogenase interconversion in intact rat heart mitochondria. Biochem J. 1981 Mar 15;194(3):721–732. doi: 10.1042/bj1940721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Haylett D. G., Jenkinson D. H. Effects of noradrenaline on potassium reflux, membrane potential and electrolyte levels in tissue slices prepared from guinea-pig liver. J Physiol. 1972 Sep;225(3):721–750. doi: 10.1113/jphysiol.1972.sp009966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Jakob A., Diem S. Metabolic responses of perfused rat livers to alpha- and beta-adrenergic agonists, glucagon and cyclic AMP. Biochim Biophys Acta. 1975 Sep 8;404(1):57–66. doi: 10.1016/0304-4165(75)90147-6. [DOI] [PubMed] [Google Scholar]
  24. Keppens S., Vandenheede J. R., De Wulf H. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase. Biochim Biophys Acta. 1977 Feb 28;496(2):448–457. doi: 10.1016/0304-4165(77)90327-0. [DOI] [PubMed] [Google Scholar]
  25. Ly S., Kim K. H. Inactivation of hepatic acetyl-CoA carboxylase by catecholamine and its agonists through the alpha-adrenergic receptors. J Biol Chem. 1981 Nov 25;256(22):11585–11590. [PubMed] [Google Scholar]
  26. Mela L. Interactions of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake. Arch Biochem Biophys. 1968 Feb;123(2):286–293. doi: 10.1016/0003-9861(68)90136-7. [DOI] [PubMed] [Google Scholar]
  27. Murphy E., Coll K., Rich T. L., Williamson J. R. Hormonal effects on calcium homeostasis in isolated hepatocytes. J Biol Chem. 1980 Jul 25;255(14):6600–6608. [PubMed] [Google Scholar]
  28. Nicholls D. G., Crompton M. Mitochondrial calcium transport. FEBS Lett. 1980 Mar 10;111(2):261–268. doi: 10.1016/0014-5793(80)80806-4. [DOI] [PubMed] [Google Scholar]
  29. Poggioli J., Berthon B., Claret M. Calcium movements in in situ mitochondria following activation of alpha-adrenergic receptors in rat liver cells. FEBS Lett. 1980 Jun 30;115(2):243–246. doi: 10.1016/0014-5793(80)81178-1. [DOI] [PubMed] [Google Scholar]
  30. Reinhart P. H., Taylor W. M., Bygrave F. L. Studies on alpha-adrenergic-induced respiration and glycogenolysis in perfused rat liver. J Biol Chem. 1982 Feb 25;257(4):1906–1912. [PubMed] [Google Scholar]
  31. Reinhart P. H., Taylor W. M., Bygrave F. L. Trifluoperazine, an inhibitor of calmodulin action, antagonises phenylephrine-induced metabolic responses and mitochondrial calcium fluxes in liver. FEBS Lett. 1980 Oct 20;120(1):71–74. doi: 10.1016/0014-5793(80)81049-0. [DOI] [PubMed] [Google Scholar]
  32. Taylor W. M., Prpić V., Exton J. H., Bygrave F. L. Stable changes to calcium fluxes in mitochondria isolated from rat livers perfused with alpha-adrenergic agonists and with glucagon. Biochem J. 1980 May 15;188(2):443–450. doi: 10.1042/bj1880443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Whiting J. A., Barritt G. J. On the mechanism by which hormones induce the release of Ca2+ from mitochondria in the liver cell. Biochem J. 1982 Jul 15;206(1):121–129. doi: 10.1042/bj2060121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Williamson J. R., Cooper R. H., Hoek J. B. Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta. 1981 Dec 30;639(3-4):243–295. doi: 10.1016/0304-4173(81)90012-4. [DOI] [PubMed] [Google Scholar]
  35. Yip B. P., Lardy H. A. The role of calcium in the stimulation of gluconeogenesis by catecholamines. Arch Biochem Biophys. 1981 Dec;212(2):370–377. doi: 10.1016/0003-9861(81)90377-5. [DOI] [PubMed] [Google Scholar]
  36. van de Werve G., Hue L., Hers H. G. Hormonal and ionic control of the glycogenolytic cascade in rat liver. Biochem J. 1977 Jan 15;162(1):135–142. doi: 10.1042/bj1620135. [DOI] [PMC free article] [PubMed] [Google Scholar]

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