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. 1983 Apr 1;211(1):13–22. doi: 10.1042/bj2110013

Isolation and characterization of human cervical-mucus glycoproteins.

I Carlstedt, H Lindgren, J K Sheehan, U Ulmsten, L Wingerup
PMCID: PMC1154324  PMID: 6409086

Abstract

Mucus glycoproteins (mucins) were extracted from human cervical pregnancy mucus by 6 M-guanidinium chloride in the presence of proteinase inhibitors. Purification was subsequently achieved by isopycnic density-gradient centrifugation in CsCl/ guanidinium chloride gradients. The purified macromolecules represented approx. 85% of the total and were devoid of nucleic acids and proteins, as judged by analytical density-gradient centrifugation, disc electrophoresis and u.v. spectroscopy. Sedimentation-velocity centrifugation revealed a single unimodal peak with S20,W 50.1S in 0.2M-NaCl and 37.0S in 6 M-guanidinium chloride. Molecular weights obtained by light-scattering were 9.7 X 10(6) and 5.9 X 10(6) in 0.2M-NaCl and 6 M-guanidinium chloride respectively. The chemical analyses were typical of those of epithelial mucins. The macromolecules contained approx. 20% (w/w) of protein, and 65% (w/w) was accounted for as carbohydrate. Serine and threonine constituted 32 mol/100 mol and proline 10 mol/100 mol of the amino acids. The major sugars found were N-acetylglucosamine (12.8%), N-acetylgalactosamine (9.7%), galactose (18.7%), sialic acid (15.0%) and fucose (7.5%).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhaskar K. R., Creeth J. M. The macromolecular properties of blood-group-specific glycoproteins. Characterization of a series of fractions obtained by density-gradient ultracentrifugation. Biochem J. 1974 Dec;143(3):669–679. doi: 10.1042/bj1430669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASASSA E. F., EISENBERG H. THERMODYNAMIC ANALYSIS OF MULTICOMPONENT SOLUTIONS. Adv Protein Chem. 1964;19:287–395. doi: 10.1016/s0065-3233(08)60191-6. [DOI] [PubMed] [Google Scholar]
  3. Carlstedt I., Sheehan J., Ulmsten U., Wingerup L. Isolation and purification of the mucin component of human cervical mucus. Adv Exp Med Biol. 1982;144:273–275. doi: 10.1007/978-1-4615-9254-9_43. [DOI] [PubMed] [Google Scholar]
  4. Chretien F. C., Engelmann P., Dubois R. The variation of the mean spinability of human cervical mucus throughout the various stages of reproductive life. Automatic measurement and statistical study. Eur J Obstet Gynecol Reprod Biol. 1979 Oct;9(5):289–297. doi: 10.1016/0028-2243(79)90078-9. [DOI] [PubMed] [Google Scholar]
  5. Clamp J. R. Chemical aspects of mucus. General considerations. Br Med Bull. 1978 Jan;34(1):25–27. doi: 10.1093/oxfordjournals.bmb.a071455. [DOI] [PubMed] [Google Scholar]
  6. Creeth J. M. Constituents of mucus and their separation. Br Med Bull. 1978 Jan;34(1):17–24. doi: 10.1093/oxfordjournals.bmb.a071454. [DOI] [PubMed] [Google Scholar]
  7. Creeth J. M. Some physical properties of mucins and their molecular origin. Mod Probl Paediatr. 1976 Oct 24;19:34–45. [PubMed] [Google Scholar]
  8. GIBBONS R. A., GLOVER F. A. The physicochemical properties of two mucoids from bovine cervical mucin. Biochem J. 1959 Oct;73:217–225. doi: 10.1042/bj0730217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godfrey J. E. The flexibility of low molecular weight double-stranded DNA as a function of length. I. Isolation andphysical characterization of seven fractions. Biophys Chem. 1976 Sep;5(3):285–299. doi: 10.1016/0301-4622(76)80041-5. [DOI] [PubMed] [Google Scholar]
  10. Hatcher V. B., Schwarzmann G. O., Jeanloz R. W., McArthur J. W. Purification, properties, and partial structure elucidation of a high-molecular-weight glycoprotein from cervical mucus of the bonnet monkey (Macaca radiata). Biochemistry. 1977 Apr 5;16(7):1518–1524. doi: 10.1021/bi00626a042. [DOI] [PubMed] [Google Scholar]
  11. Iacobelli S., Garcea N., Angeloni C. Biochemistry of cervical mucus: a comparative analysis of the secretion from preovulatory, postovulatory, and pregnancy periods. Fertil Steril. 1971 Nov;22(11):727–734. doi: 10.1016/s0015-0282(16)38582-x. [DOI] [PubMed] [Google Scholar]
  12. Jourdian G. W., Dean L., Roseman S. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem. 1971 Jan 25;246(2):430–435. [PubMed] [Google Scholar]
  13. Lindahl U. Attempted isolation of a heparin proteoglycan from bovine liver capsule. Biochem J. 1970 Jan;116(1):27–34. doi: 10.1042/bj1160027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lohmander L. S., De Luca S., Nilsson B., Hascall V. C., Caputo C. B., Kimura J. H., Heinegard D. Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1980 Jul 10;255(13):6084–6091. [PubMed] [Google Scholar]
  15. Meyer F. A., Silberberg A. Structure and function of mucus. Ciba Found Symp. 1978;(54):203–218. doi: 10.1002/9780470720356.ch10. [DOI] [PubMed] [Google Scholar]
  16. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  17. Sajdera S. W., Hascall V. C. Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures. J Biol Chem. 1969 Jan 10;244(1):77–87. [PubMed] [Google Scholar]
  18. Snary D., Allen A., Pain R. H. Conformational changes in gastric mucoproteins induced by caesium chloride and guanidinium chloride. Biochem J. 1974 Sep;141(3):641–646. doi: 10.1042/bj1410641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tanford C. Protein denaturation. Adv Protein Chem. 1968;23:121–282. doi: 10.1016/s0065-3233(08)60401-5. [DOI] [PubMed] [Google Scholar]
  20. Van Kooij R. J., Roelofs H. J., Kathmann G. A., Kramer M. F. Human cervical mucus and its mucous glycoprotein during the menstrual cycle. Fertil Steril. 1980 Sep;34(3):226–233. [PubMed] [Google Scholar]
  21. Wolf D. P., Blasco L., Khan M. A., Litt M. Human cervical mucus. IV. Viscoelasticity and sperm penetrability during the ovulatory menstrual cycle. Fertil Steril. 1978 Aug;30(2):163–169. doi: 10.1016/s0015-0282(16)43454-0. [DOI] [PubMed] [Google Scholar]
  22. Wolf D. P., Sokoloski J. E., Litt M. Composition and functioin of human cervical mucus. Biochim Biophys Acta. 1980 Jul 15;630(4):545–558. doi: 10.1016/0304-4165(80)90009-4. [DOI] [PubMed] [Google Scholar]
  23. Yurewicz E. C., Moghissi K. S. Purification of human midcycle cervical mucin and characterization of its oligosaccharides with respect to size, composition, and microheterogeneity. J Biol Chem. 1981 Nov 25;256(22):11895–11904. [PubMed] [Google Scholar]

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