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. 1983 May 1;211(2):313–318. doi: 10.1042/bj2110313

The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor.

T E Cawston, G Murphy, E Mercer, W A Galloway, B L Hazleman, J J Reynolds
PMCID: PMC1154361  PMID: 6307277

Abstract

1. Pure rabbit bone metalloproteinase inhibitor (TIMP) bound tightly to pure rabbit bone collagenase with an apparent Kd of 1.4 X 10(-10) M. 2. The molecular weight of the enzyme-inhibitor complex was found to be 54 000, but no enzyme activity could be recovered from the complex after treatment with either mercurials or proteinases. The complex thus differed from latent collagenase in terms of size, susceptibility to mercurials and behaviour on concanavalin A-Sepharose. 3. The interaction of the purified components was compared with that of crude collagenase and crude inhibitor in culture medium. Mercurial treatment partially reversed the inhibition in the crude system, but not when the purified components were used. 4. The significance of the results is discussed in relation to the extracellular control of the activity of collagenase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cawston T. E., Barrett A. J. A rapid and reproducible assay for collagenase using [1-14C]acetylated collagen. Anal Biochem. 1979 Nov 1;99(2):340–345. doi: 10.1016/s0003-2697(79)80017-2. [DOI] [PubMed] [Google Scholar]
  2. Cawston T. E., Galloway W. A., Mercer E., Murphy G., Reynolds J. J. Purification of rabbit bone inhibitor of collagenase. Biochem J. 1981 Apr 1;195(1):159–165. doi: 10.1042/bj1950159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cawston T. E., Mercer E., Tyler J. A. The activation of latent pig synovial collagenase. Biochim Biophys Acta. 1981 Jan 15;657(1):73–83. doi: 10.1016/0005-2744(81)90131-5. [DOI] [PubMed] [Google Scholar]
  4. Cawston T. E., Murphy G., Galloway W. A., Reynolds J. J. The binding of rabbit bone inhibitor of collagenase to concanavalin A--Sepharose [proceedings]. Biochem Soc Trans. 1980 Feb;8(1):112–113. doi: 10.1042/bst0080112. [DOI] [PubMed] [Google Scholar]
  5. Cawston T. E., Tyler J. A. Purification of pig synovial collagenase to high specific activity. Biochem J. 1979 Dec 1;183(3):647–656. doi: 10.1042/bj1830647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dayer J. M., Graham R., Russell G., Krane S. M. Collagenase production by rheumatoid synovial cells: stimulation by a human lymphocyte factor. Science. 1977 Jan 14;195(4274):181–183. doi: 10.1126/science.188134. [DOI] [PubMed] [Google Scholar]
  7. GREEN N. M., WORK E. Pancreatic trypsin inhibitor. II. Reaction with trypsin. Biochem J. 1953 May;54(2):347–352. doi: 10.1042/bj0540347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Galloway W. A., Murphy G., Sandy J. D., Gavrilovic J., Cawston T. E., Reynolds J. J. Purification and characterization of a rabbit bone metalloproteinase that degrades proteoglycan and other connective-tissue components. Biochem J. 1983 Mar 1;209(3):741–752. doi: 10.1042/bj2090741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murphy G., Cartwright E. C., Sellers A., Reynolds J. J. The detection and characterisation of collagenase inhibitors from rabbit tissues in culture. Biochim Biophys Acta. 1977 Aug 11;483(2):493–498. doi: 10.1016/0005-2744(77)90080-8. [DOI] [PubMed] [Google Scholar]
  10. Murphy G., Cawston T. E., Reynolds J. J. An inhibitor of collagenase from human amniotic fluid. Purification, characterization and action on metalloproteinases. Biochem J. 1981 Apr 1;195(1):167–170. doi: 10.1042/bj1950167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagase H., Cawston T. E., De Silva M., Barrett A. J. Identification of plasma kallikrein as an activator of latent collagenase in rheumatoid synovial fluid. Biochim Biophys Acta. 1982 Mar 18;702(1):133–142. doi: 10.1016/0167-4838(82)90036-x. [DOI] [PubMed] [Google Scholar]
  12. Nagase H., Jackson R. C., Brinckerhoff C. E., Vater C. A., Harris E. D., Jr A precursor form of latent collagenase produced in a cell-free system with mRNA from rabbit synovial cells. J Biol Chem. 1981 Dec 10;256(23):11951–11954. [PubMed] [Google Scholar]
  13. Nolan J. C., Ridge S. C., Oronsky A. L., Kerwar S. S. Purification and properties of a collagenase inhibitor from cultures of bovine aorta. Atherosclerosis. 1980 Jan;35(1):93–102. doi: 10.1016/0021-9150(80)90031-3. [DOI] [PubMed] [Google Scholar]
  14. Reynolds J. J., Murphy G., Sellers A., Cartwright E. A new factor that may control collagen resorption. Lancet. 1977 Aug 13;2(8033):333–335. doi: 10.1016/s0140-6736(77)91490-8. [DOI] [PubMed] [Google Scholar]
  15. Sakamoto S., Sakamoto M., Matsumoto A., Nagayama M., Glimcher M. J. Chick bone collagenase inhibitor and latency of collagenase. Biochem Biophys Res Commun. 1981 Nov 16;103(1):339–346. doi: 10.1016/0006-291x(81)91698-3. [DOI] [PubMed] [Google Scholar]
  16. Sellers A., Cartwright E., Murphy G., Reynolds J. J. Evidence that latent collagenases are enzyme-inhibitor complexes. Biochem J. 1977 May 1;163(2):303–307. doi: 10.1042/bj1630303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simpson J. W., Mailman M. L. Synthesis of a collagenase inhibitor by gingival fibroblasts in culture. Biochim Biophys Acta. 1981 Mar 18;673(3):279–285. doi: 10.1016/0304-4165(81)90336-6. [DOI] [PubMed] [Google Scholar]
  18. Stricklin G. P., Bauer E. A., Jeffrey J. J., Eisen A. Z. Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures. Biochemistry. 1977 Apr 19;16(8):1607–1615. doi: 10.1021/bi00627a013. [DOI] [PubMed] [Google Scholar]
  19. Tyler J. A., Cawston T. E. Properties of pig synovial collagenase. Biochem J. 1980 Aug 1;189(2):349–357. doi: 10.1042/bj1890349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vater C. A., Mainardi C. L., Harris E. D., Jr Inhibitor of human collagenase from cultures of human tendon. J Biol Chem. 1979 Apr 25;254(8):3045–3053. [PubMed] [Google Scholar]
  21. Welgus H. G., Stricklin G. P., Eisen A. Z., Bauer E. A., Cooney R. V., Jeffrey J. J. A specific inhibitor of vertebrate collagenase produced by human skin fibroblasts. J Biol Chem. 1979 Mar 25;254(6):1938–1943. [PubMed] [Google Scholar]
  22. Werb Z., Mainardi C. L., Vater C. A., Harris E. D., Jr Endogenous activiation of latent collagenase by rheumatoid synovial cells. Evidence for a role of plasminogen activator. N Engl J Med. 1977 May 5;296(18):1017–1023. doi: 10.1056/NEJM197705052961801. [DOI] [PubMed] [Google Scholar]

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