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. 1983 Jun 1;211(3):727–734. doi: 10.1042/bj2110727

Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae.

M Okada, S Natori
PMCID: PMC1154420  PMID: 6882366

Abstract

Three antibacterial proteins were induced when the body wall of Sarcophaga peregrina (flesh-fly) larvae was injured with a hypodermic needle. These proteins were separated and one was purified to homogeneity. The molecular weight of the purified protein was 5000 and its amino acid composition was similar to that of cecropins, which are antibacterial proteins in Hyalophora cecropia (cecropia moth) pupae. This protein was found to have bactericidal activity and to be effective at a concentration of 0.1 micrograms/ml against certain Gram-negative and Gram-positive bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIGGS J. D. Humoral immunity in lepidopterous larvae. J Exp Zool. 1958 Jun;138(1):155–188. doi: 10.1002/jez.1401380106. [DOI] [PubMed] [Google Scholar]
  2. Boman H. G., Nilsson-Faye I., Paul K., Rasmuson T., Jr Insect immunity. I. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Samia cynthia pupae. Infect Immun. 1974 Jul;10(1):136–145. doi: 10.1128/iai.10.1.136-145.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  4. Hultmark D., Engström A., Bennich H., Kapur R., Boman H. G. Insect immunity: isolation and structure of cecropin D and four minor antibacterial components from Cecropia pupae. Eur J Biochem. 1982 Sep;127(1):207–217. doi: 10.1111/j.1432-1033.1982.tb06857.x. [DOI] [PubMed] [Google Scholar]
  5. Hultmark D., Steiner H., Rasmuson T., Boman H. G. Insect immunity. Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. Eur J Biochem. 1980 May;106(1):7–16. doi: 10.1111/j.1432-1033.1980.tb05991.x. [DOI] [PubMed] [Google Scholar]
  6. Komano H., Mizuno D., Natori S. A possible mechanism of induction of insect lectin. J Biol Chem. 1981 Jul 25;256(14):7087–7089. [PubMed] [Google Scholar]
  7. Komano H., Mizuno D., Natori S. Purification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury. J Biol Chem. 1980 Apr 10;255(7):2919–2924. [PubMed] [Google Scholar]
  8. Komano H., Nozawa R., Mizuno D., Natori S. Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay. J Biol Chem. 1983 Feb 25;258(4):2143–2147. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Nakajima H., Komano H., Esumi-Kurisu M., Abe S., Yamazaki M., Natori S., Mizuno D. Induction of macrophage-mediated tumor lysis by an animal lectin, Sarcophaga peregrina agglutinin. Gan. 1982 Aug;73(4):627–632. [PubMed] [Google Scholar]
  11. Pye A. E., Boman H. G. Insect immunity. III. Purification and partial characterization of immune protein P5 from hemolymph of Hyalophora cecropia pupae. Infect Immun. 1977 Aug;17(2):408–414. doi: 10.1128/iai.17.2.408-414.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Qu Z., Steiner H., Engström A., Bennich H., Boman H. G. Insect immunity: isolation and structure of cecropins B and D from pupae of the Chinese oak silk moth, Antheraea pernyi. Eur J Biochem. 1982 Sep;127(1):219–224. doi: 10.1111/j.1432-1033.1982.tb06858.x. [DOI] [PubMed] [Google Scholar]
  13. Schmit A. R., Ratcliffe N. A. The encapsulation of foreign tissue implants in Galleria mellonella larvae. J Insect Physiol. 1977;23(2):175–184. doi: 10.1016/0022-1910(77)90027-0. [DOI] [PubMed] [Google Scholar]
  14. Scott M. T. Recognition of foreignness in invertebrates. II. In vitro studies of cockroach phagocytic haemocytes. Immunology. 1971 Nov;21(5):817–828. [PMC free article] [PubMed] [Google Scholar]
  15. Steiner H., Hultmark D., Engström A., Bennich H., Boman H. G. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature. 1981 Jul 16;292(5820):246–248. doi: 10.1038/292246a0. [DOI] [PubMed] [Google Scholar]

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