Fig. 5.

Proteomic analysis of the liver. (a–c) Volcano plots of proteins upregulated in (a) sleeve compared to control in the 2 weeks experiment, in (b) sleeve compared to control in the 8 weeks experiment and in (c) 2 weeks sleeves compared to 8 weeks sleeves. (d) Predicted regulation of transcription factor activities in the comparison of 2 weeks sleeve vs control using the decoupleR package (see supplementary text file for details). (e) PPI network of proteins upregulated in sleeve livers compared to control livers obtained from the 2-week experiment. Proteins involved in the stress/inflammation (including elevated neutrophil involvement, such as neutrophil infiltration)-associated pathways (using the (red color; GO0006950). Proteins associated with increased mortality or altered cellular aging patterns (blue color; MP 0010768 “Mortality/aging”). (a–b) minimum 2-fold regulation, p-value max. 0.05; (c) 1.5-fold level changes. PPI networks were generated using string-db.org. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)