Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1994 Jul 15;478(Pt 2):239–249. doi: 10.1113/jphysiol.1994.sp020246

In vivo voltammetric measurement of evoked extracellular dopamine in the rat basolateral amygdaloid nucleus.

P A Garris 1, R M Wightman 1
PMCID: PMC1155682  PMID: 7965845

Abstract

1. The in vivo measurement of evoked extracellular dopamine was established in the basolateral amygdaloid nucleus (BAN) using fast-scan cyclic voltammetry at carbon-fibre microelectrodes. 2. The identification of evoked extracellular dopamine in the BAN was based on anatomical, electrochemical and pharmacological criteria. Electrochemical and pharmacological evidence indicated that the species was a catecholamine. Mesencephalic sites eliciting overflow and amygdaloid sites supporting overflow correlated well with the mesoamygdaloid dopamine innervation. 3. Marked differences in the dynamics and magnitude of evoked dopamine overflow were observed in the BAN, caudate-putamen and amygdalo-striatal transition area. The results underscore the importance of making spatially resolved measurements of extracellular dopamine in the amygdala. 4. Mesoamygdaloid dopamine neurons have similar release characteristics as mesostriatal dopamine neurons but share with mesoprefrontal cortical dopamine neurons the ability to use a greater percentage of intraneuronal dopamine stores for release.

Full text

PDF
239

Images in this article

241Figure 1
on p.241

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. N. In vivo electrochemical measurements in the CNS. Prog Neurobiol. 1990;35(4):297–311. doi: 10.1016/0301-0082(90)90014-8. [DOI] [PubMed] [Google Scholar]
  2. Bannon M. J., Bunney E. B., Roth R. H. Mesocortical dopamine neurons: rapid transmitter turnover compared to other brain catecholamine systems. Brain Res. 1981 Aug 10;218(1-2):376–382. [PubMed] [Google Scholar]
  3. Bannon M. J., Roth R. H. Pharmacology of mesocortical dopamine neurons. Pharmacol Rev. 1983 Mar;35(1):53–68. [PubMed] [Google Scholar]
  4. Baur J. E., Kristensen E. W., May L. J., Wiedemann D. J., Wightman R. M. Fast-scan voltammetry of biogenic amines. Anal Chem. 1988 Jul 1;60(13):1268–1272. doi: 10.1021/ac00164a006. [DOI] [PubMed] [Google Scholar]
  5. Demarest K. T., Moore K. E. Lack of a high affinity transport system for dopamine in the median eminence and posterior pituitary. Brain Res. 1979 Aug 10;171(3):545–551. doi: 10.1016/0006-8993(79)91060-6. [DOI] [PubMed] [Google Scholar]
  6. Dietl H. Differential effects of experimentally induced blood pressure changes on the release of catecholamines in hypothalamic and limbic areas of rats. Life Sci. 1987 Jul 13;41(2):217–226. doi: 10.1016/0024-3205(87)90496-6. [DOI] [PubMed] [Google Scholar]
  7. Ewing A. G., Bigelow J. C., Wightman R. M. Direct in vivo monitoring of dopamine released from two striatal compartments in the rat. Science. 1983 Jul 8;221(4606):169–171. doi: 10.1126/science.6857277. [DOI] [PubMed] [Google Scholar]
  8. Ewing A. G., Wightman R. M. Monitoring the stimulated release of dopamine with in vivo voltammetry. II: Clearance of released dopamine from extracellular fluid. J Neurochem. 1984 Aug;43(2):570–577. doi: 10.1111/j.1471-4159.1984.tb00936.x. [DOI] [PubMed] [Google Scholar]
  9. Fallon J. H. Topographic organization of ascending dopaminergic projections. Ann N Y Acad Sci. 1988;537:1–9. doi: 10.1111/j.1749-6632.1988.tb42093.x. [DOI] [PubMed] [Google Scholar]
  10. Garris P. A., Collins L. B., Jones S. R., Wightman R. M. Evoked extracellular dopamine in vivo in the medial prefrontal cortex. J Neurochem. 1993 Aug;61(2):637–647. doi: 10.1111/j.1471-4159.1993.tb02168.x. [DOI] [PubMed] [Google Scholar]
  11. Garris P. A., Wightman R. M. Different kinetics govern dopaminergic transmission in the amygdala, prefrontal cortex, and striatum: an in vivo voltammetric study. J Neurosci. 1994 Jan;14(1):442–450. doi: 10.1523/JNEUROSCI.14-01-00442.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoffmann I. S., Talmaciu R. K., Ferro C. P., Cubeddu L. X. Sustained high release at rapid stimulation rates and reduced functional autoreceptors characterize prefrontal cortex dopamine terminals. J Pharmacol Exp Ther. 1988 Jun;245(3):761–772. [PubMed] [Google Scholar]
  13. Horn A. S. Dopamine uptake: a review of progress in the last decade. Prog Neurobiol. 1990;34(5):387–400. doi: 10.1016/0301-0082(90)90033-d. [DOI] [PubMed] [Google Scholar]
  14. Ishida Y., Hashiguchi H., Yamamoto R., Hashitani T., Ikeda T., Nishino H. Effect of intra-amygdala dopaminergic grafts on methamphetamine-induced locomotor activity, extracellular dopamine and dopamine metabolite overflow: a comparison with the effect of intra-accumbens grafts. Brain Res. 1991 May 24;549(2):342–345. doi: 10.1016/0006-8993(91)90479-f. [DOI] [PubMed] [Google Scholar]
  15. Kawagoe K. T., Garris P. A., Wiedemann D. J., Wightman R. M. Regulation of transient dopamine concentration gradients in the microenvironment surrounding nerve terminals in the rat striatum. Neuroscience. 1992 Nov;51(1):55–64. doi: 10.1016/0306-4522(92)90470-m. [DOI] [PubMed] [Google Scholar]
  16. Kilts C. D., Anderson C. M., Ely T. D., Mailman R. B. The biochemistry and pharmacology of mesoamygdaloid dopamine neurons. Ann N Y Acad Sci. 1988;537:173–187. doi: 10.1111/j.1749-6632.1988.tb42105.x. [DOI] [PubMed] [Google Scholar]
  17. Kilts C. D., Anderson C. M., Ely T. D., Nishita J. K. Absence of synthesis-modulating nerve terminal autoreceptors on mesoamygdaloid and other mesolimbic dopamine neuronal populations. J Neurosci. 1987 Dec;7(12):3961–3975. doi: 10.1523/JNEUROSCI.07-12-03961.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kilts C. D., Anderson C. M. Mesoamygdaloid dopamine neurons: differential rates of dopamine turnover in discrete amygdaloid nuclei of the rat brain. Brain Res. 1987 Jul 28;416(2):402–408. doi: 10.1016/0006-8993(87)90926-7. [DOI] [PubMed] [Google Scholar]
  19. Kilty J. E., Lorang D., Amara S. G. Cloning and expression of a cocaine-sensitive rat dopamine transporter. Science. 1991 Oct 25;254(5031):578–579. doi: 10.1126/science.1948035. [DOI] [PubMed] [Google Scholar]
  20. Kuhr W. G., Bigelow J. C., Wightman R. M. In vivo comparison of the regulation of releasable dopamine in the caudate nucleus and the nucleus accumbens of the rat brain. J Neurosci. 1986 Apr;6(4):974–982. doi: 10.1523/JNEUROSCI.06-04-00974.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Le Moal M., Simon H. Mesocorticolimbic dopaminergic network: functional and regulatory roles. Physiol Rev. 1991 Jan;71(1):155–234. doi: 10.1152/physrev.1991.71.1.155. [DOI] [PubMed] [Google Scholar]
  22. Leviel V., Charriere B., Fayada C., Guibert B. Nigroamygdaloid dopamine neurons: nigral modulation of their activity. Brain Res. 1986 Sep 3;381(2):205–214. doi: 10.1016/0006-8993(86)90069-7. [DOI] [PubMed] [Google Scholar]
  23. Lew R., Patel A., Vaughan R. A., Wilson A., Kuhar M. J. Microheterogeneity of dopamine transporters in rat striatum and nucleus accumbens. Brain Res. 1992 Jul 3;584(1-2):266–271. doi: 10.1016/0006-8993(92)90905-o. [DOI] [PubMed] [Google Scholar]
  24. May L. J., Wightman R. M. Heterogeneity of stimulated dopamine overflow within rat striatum as observed with in vivo voltammetry. Brain Res. 1989 May 22;487(2):311–320. doi: 10.1016/0006-8993(89)90835-4. [DOI] [PubMed] [Google Scholar]
  25. Mennicken F., Savasta M., Peretti-Renucci R., Feuerstein C. Autoradiographic localization of dopamine uptake sites in the rat brain with 3H-GBR 12935. J Neural Transm Gen Sect. 1992;87(1):1–14. doi: 10.1007/BF01253106. [DOI] [PubMed] [Google Scholar]
  26. Michael A. C., Ikeda M., Justice J. B., Jr Mechanisms contributing to the recovery of striatal releasable dopamine following MFB stimulation. Brain Res. 1987 Sep 22;421(1-2):325–335. doi: 10.1016/0006-8993(87)91302-3. [DOI] [PubMed] [Google Scholar]
  27. Millar J., Stamford J. A., Kruk Z. L., Wightman R. M. Electrochemical, pharmacological and electrophysiological evidence of rapid dopamine release and removal in the rat caudate nucleus following electrical stimulation of the median forebrain bundle. Eur J Pharmacol. 1985 Mar 12;109(3):341–348. doi: 10.1016/0014-2999(85)90394-2. [DOI] [PubMed] [Google Scholar]
  28. Saavedra J. M., Zivin J. Tyrosine hydroxylase and dopamine-beta-hydroxylase: distribution in discrete areas of the rat limbic system. Brain Res. 1976 Apr 9;105(3):517–524. doi: 10.1016/0006-8993(76)90597-7. [DOI] [PubMed] [Google Scholar]
  29. Scibilia R. J., Lachowicz J. E., Kilts C. D. Topographic nonoverlapping distribution of D1 and D2 dopamine receptors in the amygdaloid nuclear complex of the rat brain. Synapse. 1992 Jun;11(2):146–154. doi: 10.1002/syn.890110208. [DOI] [PubMed] [Google Scholar]
  30. Stamford J. A., Kruk Z. L., Millar J. Stimulated limbic and striatal dopamine release measured by fast cyclic voltammetry: anatomical, electrochemical and pharmacological characterisation. Brain Res. 1988 Jun 28;454(1-2):282–288. doi: 10.1016/0006-8993(88)90828-1. [DOI] [PubMed] [Google Scholar]
  31. Takada M. The A11 catecholamine cell group: another origin of the dopaminergic innervation of the amygdala. Neurosci Lett. 1990 Oct 2;118(1):132–135. doi: 10.1016/0304-3940(90)90266-c. [DOI] [PubMed] [Google Scholar]
  32. Wiedemann D. J., Kawagoe K. T., Kennedy R. T., Ciolkowski E. L., Wightman R. M. Strategies for low detection limit measurements with cyclic voltammetry. Anal Chem. 1991 Dec 15;63(24):2965–2970. doi: 10.1021/ac00024a030. [DOI] [PubMed] [Google Scholar]
  33. Wightman R. M., Amatore C., Engstrom R. C., Hale P. D., Kristensen E. W., Kuhr W. G., May L. J. Real-time characterization of dopamine overflow and uptake in the rat striatum. Neuroscience. 1988 May;25(2):513–523. doi: 10.1016/0306-4522(88)90255-2. [DOI] [PubMed] [Google Scholar]
  34. Wood P. L., Altar C. A. Dopamine release in vivo from nigrostriatal, mesolimbic, and mesocortical neurons: utility of 3-methoxytyramine measurements. Pharmacol Rev. 1988 Sep;40(3):163–187. [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES