Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1994 Aug 15;479(Pt 1):109–125. doi: 10.1113/jphysiol.1994.sp020281

Origin of delayed outward ionic current in charge movement traces from frog skeletal muscle.

C S Hui 1, W Chen 1
PMCID: PMC1155729  PMID: 7527460

Abstract

1. Non-linear membrane ionic current was studied in highly stretched cut frog twitch fibres in a double Vaseline-gap voltage clamp chamber, with the internal solution containing 0.1 mM EGTA and the external solution containing Cl- as the major anion. After the Na+ currents was abolished by TTX in the external solution and the K+ currents were suppressed by external TEA+ and Rb+ and internal Cs+, a delayed outward ionic current with a time course similar to that of the delayed rectifier current was observed during depolarization. 2. The delayed outward ionic current was resistant to 1 mM 3,4-diaminopyridine (3,4-DAP) in the external solution and was unaltered when a fraction of the internal Cs+ was replaced by K+ or Na+, suggesting that the current was not carried by cations flowing through the delayed rectifiers. 3. The delayed outward ionic current was greatly reduced by replacing the external Cl- with CH3SO3-,SO4(2-), glutamate or gluconate, indicating strongly that the current was carried by Cl- flowing through anion channels. The current was also suppressed by 1 mM external 9-anthracenecarboxylic acid (9-ACA). 4. The delayed outward ionic current was reduced by blockers of calcium-dependent Cl- channels, such as SITS and frusemide (furosemide), in a dose- and voltage-dependent manner and by increasing intracellular [EGTA] to 20 mM, suggesting that part of the Cl- current in the muscle fibres could be calcium dependent. 5. The total Cl- current could be dissected into calcium-dependent and calcium-independent components. Each component accounted for roughly half of the total Cl- current. The maximum slope conductance of the calcium-dependent Cl- channels was 60.9 +/- 6.0 microS microF-1 (mean +/- S.E.M., n = 4).

Full text

PDF
109

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akasu T., Nishimura T., Tokimasa T. Calcium-dependent chloride current in neurones of the rabbit pelvic parasympathetic ganglia. J Physiol. 1990 Mar;422:303–320. doi: 10.1113/jphysiol.1990.sp017985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bader C. R., Bertrand D., Schlichter R. Calcium-activated chloride current in cultured sensory and parasympathetic quail neurones. J Physiol. 1987 Dec;394:125–148. doi: 10.1113/jphysiol.1987.sp016863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barish M. E. A transient calcium-dependent chloride current in the immature Xenopus oocyte. J Physiol. 1983 Sep;342:309–325. doi: 10.1113/jphysiol.1983.sp014852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bretag A. H., Dawe S. R., Moskwa A. G. Chemically induced myotonia in amphibia. Nature. 1980 Aug 7;286(5773):625–626. doi: 10.1038/286625a0. [DOI] [PubMed] [Google Scholar]
  5. Bretag A. H. Muscle chloride channels. Physiol Rev. 1987 Apr;67(2):618–724. doi: 10.1152/physrev.1987.67.2.618. [DOI] [PubMed] [Google Scholar]
  6. Byrne N. G., Large W. A. Action of noradrenaline on single smooth muscle cells freshly dispersed from the rat anococcygeus muscle. J Physiol. 1987 Aug;389:513–525. doi: 10.1113/jphysiol.1987.sp016669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cahalan M. D., Lewis R. S. Role of potassium and chloride channels in volume regulation by T lymphocytes. Soc Gen Physiol Ser. 1988;43:281–301. [PubMed] [Google Scholar]
  8. Chandler W. K., Hui C. S. Membrane capacitance in frog cut twitch fibers mounted in a double vaseline-gap chamber. J Gen Physiol. 1990 Aug;96(2):225–256. doi: 10.1085/jgp.96.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chen W., Hui C. S. Differential blockage of charge movement components in frog cut twitch fibres by nifedipine. J Physiol. 1991 Dec;444:579–603. doi: 10.1113/jphysiol.1991.sp018895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chen W., Hui C. S. Existence of Q gamma in frog cut twitch fibers with little Q beta. Biophys J. 1991 Feb;59(2):503–507. doi: 10.1016/S0006-3495(91)82243-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans M. G., Marty A. Calcium-dependent chloride currents in isolated cells from rat lacrimal glands. J Physiol. 1986 Sep;378:437–460. doi: 10.1113/jphysiol.1986.sp016229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Evans M. G., Marty A., Tan Y. P., Trautmann A. Blockage of Ca-activated Cl conductance by furosemide in rat lacrimal glands. Pflugers Arch. 1986 Jan;406(1):65–68. doi: 10.1007/BF00582955. [DOI] [PubMed] [Google Scholar]
  13. Findlay I., Petersen O. H. Acetylcholine stimulates a Ca2+-dependent C1- conductance in mouse lacrimal acinar cells. Pflugers Arch. 1985 Mar;403(3):328–330. doi: 10.1007/BF00583609. [DOI] [PubMed] [Google Scholar]
  14. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HUTTER O. F., NOBLE D. The chloride conductance of frog skeletal muscle. J Physiol. 1960 Apr;151:89–102. [PMC free article] [PubMed] [Google Scholar]
  16. Hansen M., Skydsgaard J. M. Stilbene disulphonates inhibit apparently separate chloride transporters in skeletal muscle of Rana temporaria. J Physiol. 1992 Mar;448:383–395. doi: 10.1113/jphysiol.1992.sp019047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hui C. S., Chandler W. K. Intramembranous charge movement in frog cut twitch fibers mounted in a double vaseline-gap chamber. J Gen Physiol. 1990 Aug;96(2):257–297. doi: 10.1085/jgp.96.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hui C. S., Chandler W. K. Q beta and Q gamma components of intramembranous charge movement in frog cut twitch fibers. J Gen Physiol. 1991 Sep;98(3):429–464. doi: 10.1085/jgp.98.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hui C. S., Chen W. Effects of conditioning depolarization and repetitive stimulation on Q beta and Q gamma charge components in frog cut twitch fibers. J Gen Physiol. 1992 Jun;99(6):1017–1043. doi: 10.1085/jgp.99.6.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hui C. S., Chen W. Evidence for the non-existence of a negative phase in the hump charge movement component (I gamma) in Rana temporaria. J Physiol. 1994 Jan 15;474(2):275–282. doi: 10.1113/jphysiol.1994.sp020020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hui C. S., Chen W. Separation of Q beta and Q gamma charge components in frog cut twitch fibers with tetracaine. Critical comparison with other methods. J Gen Physiol. 1992 Jun;99(6):985–1016. doi: 10.1085/jgp.99.6.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Hui C. S. Comparison of charge movement components in intact and cut twitch fibers of the frog. Effects of stretch and temperature. J Gen Physiol. 1991 Aug;98(2):287–314. doi: 10.1085/jgp.98.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hui C. S. D600 binding sites on voltage-sensors for excitation-contraction coupling in frog skeletal muscle are intracellular. J Muscle Res Cell Motil. 1990 Dec;11(6):471–488. doi: 10.1007/BF01745215. [DOI] [PubMed] [Google Scholar]
  24. Hui C. S. Factors affecting the appearance of the hump charge movement component in frog cut twitch fibers. J Gen Physiol. 1991 Aug;98(2):315–347. doi: 10.1085/jgp.98.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Irving M., Maylie J., Sizto N. L., Chandler W. K. Intrinsic optical and passive electrical properties of cut frog twitch fibers. J Gen Physiol. 1987 Jan;89(1):1–40. doi: 10.1085/jgp.89.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Korn S. J., Weight F. F. Patch-clamp study of the calcium-dependent chloride current in AtT-20 pituitary cells. J Neurophysiol. 1987 Dec;58(6):1431–1451. doi: 10.1152/jn.1987.58.6.1431. [DOI] [PubMed] [Google Scholar]
  27. Kovacs L., Rios E., Schneider M. F. Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye. J Physiol. 1983 Oct;343:161–196. doi: 10.1113/jphysiol.1983.sp014887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mayer M. L. A calcium-activated chloride current generates the after-depolarization of rat sensory neurones in culture. J Physiol. 1985 Jul;364:217–239. doi: 10.1113/jphysiol.1985.sp015740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Miledi R. A calcium-dependent transient outward current in Xenopus laevis oocytes. Proc R Soc Lond B Biol Sci. 1982 Jul 22;215(1201):491–497. doi: 10.1098/rspb.1982.0056. [DOI] [PubMed] [Google Scholar]
  30. Owen D. G., Segal M., Barker J. L. A Ca-dependent Cl- conductance in cultured mouse spinal neurones. Nature. 1984 Oct 11;311(5986):567–570. doi: 10.1038/311567a0. [DOI] [PubMed] [Google Scholar]
  31. Pacaud P., Loirand G., Lavie J. L., Mironneau C., Mironneau J. Calcium-activated chloride current in rat vascular smooth muscle cells in short-term primary culture. Pflugers Arch. 1989 Apr;413(6):629–636. doi: 10.1007/BF00581813. [DOI] [PubMed] [Google Scholar]
  32. Rogawski M. A., Inoue K., Suzuki S., Barker J. L. A slow calcium-dependent chloride conductance in clonal anterior pituitary cells. J Neurophysiol. 1988 Jun;59(6):1854–1870. doi: 10.1152/jn.1988.59.6.1854. [DOI] [PubMed] [Google Scholar]
  33. Woodbury J. W., Miles P. R. Anion conductance of frog muscle membranes: one channel, two kinds of pH dependence. J Gen Physiol. 1973 Sep;62(3):324–353. doi: 10.1085/jgp.62.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zygmunt A. C., Gibbons W. R. Properties of the calcium-activated chloride current in heart. J Gen Physiol. 1992 Mar;99(3):391–414. doi: 10.1085/jgp.99.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES