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. 1994 Sep 1;479(Pt 2):199–205. doi: 10.1113/jphysiol.1994.sp020288

A novel voltage-dependent cation current in rat neocortical neurones.

C Alzheimer 1
PMCID: PMC1155739  PMID: 7528275

Abstract

1. Using the whole-cell configuration of the patch-clamp technique, an unexpected voltage-dependent cation current (Icat) was recorded from acutely isolated rat neocortical neurones, the Na+, K+ and Ca2+ currents of which were pharmacologically suppressed. 2. Icat was activated at potentials more positive than -45 mV, displayed outward rectification, and deactivated with a slow voltage-dependent time course causing prominent inward tail currents. 3. Activation of Icat was not dependent on Ca2+ influx or increases in cytosolic Ca2+, since it was not abolished by inorganic Ca2+ channel blockers or by internal Ca2+ chelators. 4. Icat was reduced by tetraethylammonium at high concentrations, but not by 4-amino-pyridine, and proved to be insensitive to cation channel blockers such as Cs+, amiloride or gadolinium. 5. Ion substitution experiments revealed that the channel producing Icat was permeable to a number of monovalent cations, including K+, Cs+, Na+ and choline+, but not to the Cl-anion. 6. The features of Icat suggest that, in electrically active neurones, it should play a role in both the initial repolarization of membrane potential after strong depolarization and the generation of depolarizing after-potential.

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Selected References

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