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. 1994 Dec 1;481(Pt 2):357–369. doi: 10.1113/jphysiol.1994.sp020445

Kinetic separation of charge movement components in intact frog skeletal muscle.

C L Huang 1
PMCID: PMC1155935  PMID: 7738831

Abstract

1. Procedures for a complete charge movement separation employed a combination of its steady-state inactivation and activation properties in intact frog skeletal muscle fibres in gluconate-containing solutions. 2. Holding potential shifts from -70 to -50 mV reduced the total charge available between -90 and -20 mV from 16.76 +/- 1.70 nC microF-1 (mean +/- S.E.M.; n = 4 fibres) to 9.25 +/- 1.43 nC microF-1 without significant loss of tetracaine-resistant charge (q beta). 3. The steady-state and kinetic properties of tetracaine-sensitive charge (q gamma) persisted through holding potential changes from -90 to -70 mV in the presence of gluconate and generally resembled activation properties established hitherto in sulphate-containing solutions. 4. Further holding potential displacement to -50 mV abolished q gamma charge movements and depressed the charge-voltage curve. 5. Test voltage steps applied from a -70 mV prepulse level gave rapid monotonic q beta decays and similarly depressed activation functions in 2 mM tetracaine unchanged by holding potential shifts between -70 and -50 mV. 6. The isolated 'on' q gamma charge movements, I(t), always included early transients that preceded any prolonged charging phases and which increased with depolarization. They decayed to stable baselines in the absence of prolonged time-dependent or inward-current phases and yielded integrals, Q(t), that monotonically increased with test voltage. 7. 'Off' steps always elicited rapid monotonic q gamma decays that fully returned the 'on' charge. 8. 'On' and 'off' q gamma currents, I(t), following voltage steps from fixed conditioning to varying test levels mapped onto topologically distinct higher-order phase-plane trajectories, I(Q), that steeply varied with test voltage. 9. In contrast, voltage steps to fixed test potentials of either -70 or -20 mV elicited identical q gamma phase-plane trajectories independent of prepulse history. 10. The q gamma current thus reflects an independent, capacitative process driven uniquely by higher-order dependences upon charge distribution, Q(t), and test voltage, V(t), autonomous of prepulse history or time, t.

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Selected References

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