Figure 6. Specificity determinants and context effects in WW domain-binding peptides.

(A) Scatterplot of z-scores (median, n = 6) for peptides tested for YAP^WW1 and NEDD4^WW3 binding.

(B) Sequences and binding z-scores (median, n = 6) for 22 peptides, sorted by YAP^WW1 binding strength. The core [LP]PxY motif is colored red, and Pro residues at p+1 are colored blue.

(C) Logos showing average sequence preferences for each WW domain.

(D) Logos comparing preferences at each peptide position in the context of multiple parent peptides (identified at right for each WW domain). At bottom, plum and tan bars mark the distinct type 1 and 2 preference patterns summarized in panel (E).

(E) Logos showing distinct YAP^WW1 preferences for type 1 and 2 peptides.

(F) Sequences of the type 1 and 2 peptides that contribute to the logos in panel (E). Yellow highlighting and blue font mark Pro residues at non-core positions in type 2 peptides.

(G) AARDC3 peptide bound to the NEDD4^WW3 domain (PDB ID: 4n7h) [72]. Hydrophobic regions on the NEDD4^WW3 surface are colored yellow [105].