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. 1995 Aug 15;487(Pt 1):17–36. doi: 10.1113/jphysiol.1995.sp020858

The multi-ion nature of the cGMP-gated channel from vertebrate rods.

F Sesti 1, E Eismann 1, U B Kaupp 1, M Nizzari 1, V Torre 1
PMCID: PMC1156596  PMID: 7473247

Abstract

1. Native cGMP-gated channels were studied in rod outer segments of the larval tiger salamander, Ambystoma tigrinum. The alpha-subunit of the cGMP-gated channel, here referred to as the wild type (WT), and mutant channels were heterologously expressed in Xenopus laevis oocytes. These channels were studied in excised membrane patches in the inside-out configuration and were activated by the addition of 100 or 500 microM cGMP. The current carried by monovalent cations was measured under voltage-clamp conditions. 2. In the presence of 110 mM Na+ in the extracellular medium and different amounts of Na+ in the intracellular medium, the I-V relations of the native channel could be described by a single-site model with a profile of Gibbs free energy with two barriers and a well. A similar result was obtained in the presence of 110 mM Li+ in the extracellular medium and different amounts of Li+ in the intracellular medium. The well depth was 1.4RT (where R is the gas constant and T is the absolute temperature) for both Li+ and Na+. 3. The I-V relations of the native channel in the presence of 110 mM Na+ on one side of the membrane and 110 mM Li+ on the other side could not be described by the same single-site model with identical values of barriers and well obtained in the presence of Li+ or Na+ alone: the well for Li+ had to be at least 4RT. 4. In the presence of mixtures of 110 mM Li+ and Cs+ on the cytoplasmic side of the membrane, an anomalous mole fraction effect was observed both in the native and the WT channel. No anomalous behaviour was seen in the presence of Li(+)-Na+ and Li(+)-NH4+ mixtures. 5. The anomalous mole fraction effect with mixtures of Li+ and Cs+ was not observed in the channel where glutamate 363 was mutated to a glutamine (E363Q) or an asparagine (E363N). When glutamate 363 was mutated to an aspartate (E363D), the anomalous mole fraction effect with mixtures of Li+ and Cs+ was still observed, although significantly reduced. 6. When lysine 346, arginine 369, aspartate 370 and glutamate 372 were neutralized by mutation to glutamine, the ion permeation through the mutant channels and the WT channel had largely similar properties.(ABSTRACT TRUNCATED AT 400 WORDS)

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Selected References

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  1. Almers W., McCleskey E. W. Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore. J Physiol. 1984 Aug;353:585–608. doi: 10.1113/jphysiol.1984.sp015352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong C. M., Neyton J. Ion permeation through calcium channels. A one-site model. Ann N Y Acad Sci. 1991;635:18–25. doi: 10.1111/j.1749-6632.1991.tb36477.x. [DOI] [PubMed] [Google Scholar]
  3. Baylor D. A., Nunn B. J. Electrical properties of the light-sensitive conductance of rods of the salamander Ambystoma tigrinum. J Physiol. 1986 Feb;371:115–145. doi: 10.1113/jphysiol.1986.sp015964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bormann J., Hamill O. P., Sakmann B. Mechanism of anion permeation through channels gated by glycine and gamma-aminobutyric acid in mouse cultured spinal neurones. J Physiol. 1987 Apr;385:243–286. doi: 10.1113/jphysiol.1987.sp016493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bönigk W., Altenhofen W., Müller F., Dose A., Illing M., Molday R. S., Kaupp U. B. Rod and cone photoreceptor cells express distinct genes for cGMP-gated channels. Neuron. 1993 May;10(5):865–877. doi: 10.1016/0896-6273(93)90202-3. [DOI] [PubMed] [Google Scholar]
  6. Chen T. Y., Peng Y. W., Dhallan R. S., Ahamed B., Reed R. R., Yau K. W. A new subunit of the cyclic nucleotide-gated cation channel in retinal rods. Nature. 1993 Apr 22;362(6422):764–767. doi: 10.1038/362764a0. [DOI] [PubMed] [Google Scholar]
  7. Colamartino G., Menini A., Torre V. Blockage and permeation of divalent cations through the cyclic GMP-activated channel from tiger salamander retinal rods. J Physiol. 1991;440:189–206. doi: 10.1113/jphysiol.1991.sp018703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eismann E., Müller F., Heinemann S. H., Kaupp U. B. A single negative charge within the pore region of a cGMP-gated channel controls rectification, Ca2+ blockage, and ionic selectivity. Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1109–1113. doi: 10.1073/pnas.91.3.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Furman R. E., Tanaka J. C. Monovalent selectivity of the cyclic guanosine monophosphate-activated ion channel. J Gen Physiol. 1990 Jul;96(1):57–82. doi: 10.1085/jgp.96.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goulding E. H., Tibbs G. R., Liu D., Siegelbaum S. A. Role of H5 domain in determining pore diameter and ion permeation through cyclic nucleotide-gated channels. Nature. 1993 Jul 1;364(6432):61–64. doi: 10.1038/364061a0. [DOI] [PubMed] [Google Scholar]
  11. Guy H. R., Durell S. R., Warmke J., Drysdale R., Ganetzky B. Similarities in amino acid sequences of Drosophila eag and cyclic nucleotide-gated channels. Science. 1991 Nov 1;254(5032):730–730. doi: 10.1126/science.1658932. [DOI] [PubMed] [Google Scholar]
  12. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  13. Heginbotham L., Abramson T., MacKinnon R. A functional connection between the pores of distantly related ion channels as revealed by mutant K+ channels. Science. 1992 Nov 13;258(5085):1152–1155. doi: 10.1126/science.1279807. [DOI] [PubMed] [Google Scholar]
  14. Herlitze S., Koenen M. A general and rapid mutagenesis method using polymerase chain reaction. Gene. 1990 Jul 2;91(1):143–147. doi: 10.1016/0378-1119(90)90177-s. [DOI] [PubMed] [Google Scholar]
  15. Hess P., Tsien R. W. Mechanism of ion permeation through calcium channels. 1984 May 31-Jun 6Nature. 309(5967):453–456. doi: 10.1038/309453a0. [DOI] [PubMed] [Google Scholar]
  16. Karpen J. W., Zimmerman A. L., Stryer L., Baylor D. A. Gating kinetics of the cyclic-GMP-activated channel of retinal rods: flash photolysis and voltage-jump studies. Proc Natl Acad Sci U S A. 1988 Feb;85(4):1287–1291. doi: 10.1073/pnas.85.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kaupp U. B., Niidome T., Tanabe T., Terada S., Bönigk W., Stühmer W., Cook N. J., Kangawa K., Matsuo H., Hirose T. Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel. Nature. 1989 Dec 14;342(6251):762–766. doi: 10.1038/342762a0. [DOI] [PubMed] [Google Scholar]
  18. Kim M. S., Morii T., Sun L. X., Imoto K., Mori Y. Structural determinants of ion selectivity in brain calcium channel. FEBS Lett. 1993 Mar 1;318(2):145–148. doi: 10.1016/0014-5793(93)80009-j. [DOI] [PubMed] [Google Scholar]
  19. Kuo C. C., Hess P. Characterization of the high-affinity Ca2+ binding sites in the L-type Ca2+ channel pore in rat phaeochromocytoma cells. J Physiol. 1993 Jul;466:657–682. [PMC free article] [PubMed] [Google Scholar]
  20. Kuo C. C., Hess P. Ion permeation through the L-type Ca2+ channel in rat phaeochromocytoma cells: two sets of ion binding sites in the pore. J Physiol. 1993 Jul;466:629–655. [PMC free article] [PubMed] [Google Scholar]
  21. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Menini A. Currents carried by monovalent cations through cyclic GMP-activated channels in excised patches from salamander rods. J Physiol. 1990 May;424:167–185. doi: 10.1113/jphysiol.1990.sp018061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Menini A., Rispoli G., Torre V. The ionic selectivity of the light-sensitive current in isolated rods of the tiger salamander. J Physiol. 1988 Aug;402:279–300. doi: 10.1113/jphysiol.1988.sp017204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Miller J. L., Picones A., Korenbrot J. I. Differences in transduction between rod and cone photoreceptors: an exploration of the role of calcium homeostasis. Curr Opin Neurobiol. 1994 Aug;4(4):488–495. doi: 10.1016/0959-4388(94)90048-5. [DOI] [PubMed] [Google Scholar]
  25. Nizzari M., Sesti F., Giraudo M. T., Virginio C., Cattaneo A., Torre V. Single-channel properties of cloned cGMP-activated channels from retinal rods. Proc Biol Sci. 1993 Oct 22;254(1339):69–74. doi: 10.1098/rspb.1993.0128. [DOI] [PubMed] [Google Scholar]
  26. Picco C., Menini A. The permeability of the cGMP-activated channel to organic cations in retinal rods of the tiger salamander. J Physiol. 1993 Jan;460:741–758. doi: 10.1113/jphysiol.1993.sp019497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Root M. J., MacKinnon R. Identification of an external divalent cation-binding site in the pore of a cGMP-activated channel. Neuron. 1993 Sep;11(3):459–466. doi: 10.1016/0896-6273(93)90150-p. [DOI] [PubMed] [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sesti F., Straforini M., Lamb T. D., Torre V. Gating, selectivity and blockage of single channels activated by cyclic GMP in retinal rods of the tiger salamander. J Physiol. 1994 Jan 15;474(2):203–222. doi: 10.1113/jphysiol.1994.sp020014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yang J., Ellinor P. T., Sather W. A., Zhang J. F., Tsien R. W. Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels. Nature. 1993 Nov 11;366(6451):158–161. doi: 10.1038/366158a0. [DOI] [PubMed] [Google Scholar]
  31. Zimmerman A. L., Baylor D. A. Cation interactions within the cyclic GMP-activated channel of retinal rods from the tiger salamander. J Physiol. 1992 Apr;449:759–783. doi: 10.1113/jphysiol.1992.sp019112. [DOI] [PMC free article] [PubMed] [Google Scholar]

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