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. 1993 Feb;13(1):59–68. doi: 10.1007/BF00712989

Dendrotoxin blocks a class of potassium channels that are opened by inhibitory presynaptic modulators in rat cortical synaptosomes and slices

Gabor Zoltay 1, Jack R Cooper 1,✉
PMCID: PMC11566752  PMID: 8458063

Abstract

  1. Rat cortical synaptosomes were prelabeled with radioactive acetylcholine and the release induced by veratridine was determined in the absence and presence of the inhibitory presynaptic modulators, 2-chloroadenosine, carbamylcholine, clonidine, and morphine. All four agents inhibited the evoked release of acetylcholine and this inhibition was reversed by dendrotoxin.

  2. Using perfused cortical slices and an extracellular K-sensitive electrode, all modulators again increased K efflux that was blocked by dendrotoxin. In contrast, glybenclamide and tetraethylammonium did not block the modulatorinduced efflux.

Key words: Dendrotoxin, 2-chloroadenosine, carbamylcholine, morphine, clonidine, K channels, presynaptic modulation

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