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. 1993 Feb;13(1):15–23. doi: 10.1007/BF00712986

Increased production of endothelins in the hippocampus of stroke-prone spontaneously hypertensive rats following transient forebrain ischemia: Histochemical evidence

Kimihiro Yamashita 1,✉, Yasufumi Kataoka 1, Masami Niwa 1, Kazuto Shigematsu 2, Akihiko Himeno 3, Shuichi Koizumi 4, Kohtaro Taniyama 1
PMCID: PMC11566809  PMID: 8458060

Abstract

  1. The effect of transient forebrain ischemia on endothelin-1 (ET-1) and endothelin-3 (ET-3) production in the hippocampus of stroke-prone spontaneously hypertensive rats (SHRSPs) was investigated using immunohistochemical techniques.

  2. In SHRSPs subjected to 10-min bilateral carotid occlusion, neuronal degeneration in the CA1 pyramidal cell layer of the hippocampus was detectable at 4 days and remarkable at 7 days after reperfusion.

  3. Coinciding with neuronal degeneration, ET-1- and ET-3-like immunoreactivities were intense in the CA1 pyramidal-cell layer, the stratum lacunosum moleculare, and the CA4 subfield of the hippocampus. Almost all of the immunostained cells had morphological characteristics of astrocytes.

  4. The possibility that ET has a role in the development of neuronal cell death following transient forebrain ischemia warrants further attention.

Key words: endothelin-1, endothelin-3, transient forebrain ischemia, delayed neuronal death, hippocampus, stroke-prone spontaneously hypertensive rat, immunohistochemistry

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