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. 1990 Jun;10(2):237–255. doi: 10.1007/BF00734577

Functional expression of dihydropyridine-insensitive calcium channels during PC12 cell differentiation by nerve growth factor (NGF), oncogenic ras, or src tyrosine kinase

Dianne M Rausch 1,✉, Deborah L Lewis 2, Jeffrey L Barker 2, Lee E Eiden 1
PMCID: PMC11567337  PMID: 2163753

Abstract

  1. Recombinant retroviruses were used to introduce a temperature-sensitive v-src gene and oncogenic c-Ha-ras into PC12 cells, and stable cell lines expressing these genes were established.

  2. As previously reported, expression of v-src (Alemaet al., 1985) or c-Ha-ras (Nodaet al., 1985) in PC12 cells results in neurite outgrowth resembling that induced by NGF. We report here that v-src but not oncogenic c-Ha-ras induces a stable morphologic neuronal differentiation similar to treatment with NGF. Oncogenic c-Ha-ras-induced neurite outgrowth is not stable with long-term culture, rather the cells revert to an undifferentiated morphology with altered cell cycle kinetics.

  3. The stable neuronal phenotype induced by v-src and NGF is characterized by the functional expression of dihydropyridine-insensitive calcium currents.

Key words: oncogene, v-src, c-Ha-ras, PC12, neural differentiation, calcium channel

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