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. 1992 Apr;12(2):163–175. doi: 10.1007/BF00713370

Characteristics of C6 glioma cells overexpressing a gap junction protein

C C G Naus 1,✉, D Zhu 2, S D L Todd 2, G M Kidder 2
PMCID: PMC11567348  PMID: 1318167

Abstract

  1. C6 glioma cells transfected with connexin43 cDNA display a dramatic increase in the level of connexin43 mRNA and protein.

  2. This overexpression of connexin43 is evident at the cellular level, as revealed within situ hybridization and immunocytochemistry. Transfection with connexin43 cDNA also induced actin stress fibers in these glioma cells.

  3. Although we observed up to a 50-fold increase in the level of connexin43 mRNA following transfection, virtually all of this mRNA was present in the polysomal fraction.

  4. Overexpression of connexin43 mRNA did not appear to compete with other cellular mRNAs for access to the translational machinery.

  5. It is likely that the reduced proliferation rate of the transfected cells, reported earlier, is due to enhanced connexin43 expression and intercellular coupling.

Key words: connexin43, transfection, mRNA translation, in situ hybridization, Western blot, immunocytochemistry

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