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. 1987 Dec;7(4):381–390. doi: 10.1007/BF00733790

Alteration of the acetylcholine response by intra- and extracellular serotonin application in intracellularly perfused neurons ofLymnaea stagnalis

Tigran M Turpaev 1, Olga P Yurchenko 1, Nikita G Grigoriev 1
PMCID: PMC11567355  PMID: 2837328

Abstract

  1. The effect of serotonin on the acetylcholine (ACh) response has been studied by means of voltage clamp and intracellular perfusion in unidentified isolated neurons from parietal and visceral ganglia ofLymnaea stagnalis.

  2. In most cells studied serotonin added to the internal or external solution decreases the response to ACh.

  3. In other neurons serotonin added to the intracellular solution increases the response to ACh; when it is added extracellularly it produces the opposite effect on the same cells.

  4. The decreasing effect of serotonin on ACh currents is mimicked by cyproheptadine, an antagonist of serotonin receptors, and by the intracellular application of cyclic AMP (cAMP) forskolin.

  5. The enhancing effect of intracellularly applied serotonin on ACh currents is blocked by cyproheptadine and is not obtained by the intracellular administration of cAMP and forskolin. In some cells the enhancing effect of serotonin appears after forskolin.

  6. The results suggest a modulating effect of serotonin on cholinergic synaptic transmission in the nervous system of mollusks. The possible existence of intracellular serotonin receptors is discussed.

Key words: gastropod neurons, intracellular perfusion, voltage clamp, serotonin, acetylcholine, forskolin, cyclic AMP, cyproheptadine, intracellular neurotransmitter receptors

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