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. 1990 Sep;10(3):337–350. doi: 10.1007/BF00711179

Identification and characterization of the beta-adrenergic receptor on neuroblastoma × glioma hybrid NG108-15 cells

A Ghahary 1, K W Cheng 1,✉
PMCID: PMC11567641  PMID: 2174741

Abstract

  1. Using [3H]DHA and unlabeledl-alprenolol, a substantial amount of over 64% specific binding ofβ-adrenergic receptor has been identified on the neuroblastoma × glioma hybrid NG108-15 cell, which has been proven to display numerous functional characteristics of intact neurons.

  2. Beta-adrenergic receptor binding on intact NG108-15 cells does not change significantly upon morphological differentiation, induced by 1 mM dibutyryl cyclic AMP (dBcAMP).

  3. The [3H]DHA binding on intact NG108-15 cells is rapid, saturable, and reversible, having at 1/2 of 1.0 min for association and 3.5 min for dissociation.

  4. The affinity constant (K d) and maximum binding capacity (B max) for binding of [3H]DHA toβ-adrenergic receptors on NG108-15 cells have been estimated by Scatchard plot analysis to be 2.5 and 0.23 nM, respectively. Further analysis indicates a single class of receptors for [3HDHA binding on NG108-15 cells.

  5. Studies on kinetic properties have revealed on-rate (K + 1) and off-rate (K - 1) constants of 0.7 × 10−9 M min−1 and 0.19 min−1, respectively. Further, the IC50 value and inhibition constant (K i) for unlabeledl-alprenolol to inhibit [3HDHA binding on NG108-15 cells have been estimated to be 10−5 and 8.9 × 10−6 M, respectively.

  6. The rank-order potency of catecholamine agonists, (−)ISO > (+)ISO > EPI > NE, reveals the presence of type 2 receptor for theβ-adrenergic binding on both differentiated and undifferentiated NG108-15 cells.

  7. The present study indicates that the clonal neuroblastoma × glioma hydbrid NG108-15 cell line possesses substantial amounts ofβ-adrenergic receptors with characteristics similar to those on neuronal cells.

Key words: β-adrenergic receptor, neuroblastoma, neuronal culture

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