Figure 6.

Pathways significantly affected by iRBC exposure in HMBECs

(A) The endosomal signaling pathway was affected by co-incubation with ring stage iRBCs and shear stress (1.5 dyne/cm²), as demonstrated by mRNA-sequencing.

(B) The CXC subfamily signaling pathway was affected by co-incubation with ring stage iRBCs and shear stress 1.5 dyne/cm², with six genes downregulated and one gene upregulated.

(C) Co-incubation with ring stage iRBCs affected expression of genes encoding tight unction components in HBMECs, with most genes downregulated in HBMEC^1.5+Rings compared with HBMEC^1.5+RBCs.