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. 2024 Oct 5;10(21):e39031. doi: 10.1016/j.heliyon.2024.e39031

Fig. 5.

Fig. 5

Que inhibits mitochondrial ROS production via Sirt3/SOD2 signaling and suppresses I/R-induced cardiomyocyte apoptosis. (A) Western blot analysis of Sirt3 transfection efficiency. (B) Detection of cellular Sirt3, SOD2 and AC-SOD2 expression by Western blot analysis. (C) Mitochondrial ROS levels. (D) Cellular ATP levels. (E) Mitochondrial membrane potential. (F–I) Changes in oxidative stress indicators. (J) TUNEL assay to detect apoptosis (scale bar = 100 μm). Western blot analysis of the expression of apoptosis-related proteins (K) and Cyto-c (L). (M − N) LDH and CK levels in cell supernatants. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. NC; △P < 0.05, △△P < 0.01, △△△P < 0.001 vs. H/R; #P < 0.05, ##P < 0.01, ###P < 0.01 vs. H/R + si-Sirt3, n = 3. For the uncropped protein band plot, please see Fig. 5A,B,K,L in the Supplementary Material.