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. 1995 Jan 15;482(Pt 2):247–258. doi: 10.1113/jphysiol.1995.sp020514

Factors controlling changes in intracellular Ca2+ concentration produced by noradrenaline in rat mesenteric artery smooth muscle cells.

I Baró 1, D A Eisner 1
PMCID: PMC1157725  PMID: 7714820

Abstract

1. The intracellular Ca2+ concentration ([Ca2+]i) was measured in mesenteric artery smooth muscle cells using the fluorescent indicator indo-1. 2. Noradrenaline (1-10 microM) produced a transient increase in [Ca2+]i. This response was unaffected by the removal of external calcium suggesting that the bulk of the increase in [Ca2+]i produced by noradrenaline is due to release from an intracellular store. 3. The maintained application of caffeine (10 mM) produced a transient rise in [Ca2+]i. The rate of relaxation was slower than that of the noradrenaline response. If caffeine was removed at the peak of the rise in [Ca2+]i then [Ca2+]i recovered more quickly than was the case in both the maintained response to noradrenaline and that to caffeine. 4. In the presence of noradrenaline, caffeine or thapsigargin elevated [Ca2+]i. However, if thapsigargin or caffeine was added first, the subsequent application of noradrenaline did not increase [Ca2+]i, suggesting that only part of the caffeine-sensitive store is sensitive to noradrenaline. 5. The recovery of [Ca2+]i during the application of caffeine was unaffected by the removal of external sodium suggesting that Na+-Ca2+ exchange is not important in the reduction in [Ca2+]i. The addition of lanthanum (1 mM) did, however, greatly slow [Ca2+]i recovery. 6. We conclude that the three major factors responsible for removing Ca2+ ions from the cytoplasm are: (i) a caffeine- and noradrenaline-sensitive store (43%), (ii) a caffeine-sensitive but noradrenaline-insensitive store (36%), and (iii) a sarcolemmal Ca(2+)-ATPase (16%). Finally, a 5% contribution remains to be accounted for.

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Selected References

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  1. Ashida T., Blaustein M. P. Regulation of cell calcium and contractility in mammalian arterial smooth muscle: the role of sodium-calcium exchange. J Physiol. 1987 Nov;392:617–635. doi: 10.1113/jphysiol.1987.sp016800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baró I., Eisner D. A. The effects of thapsigargin on [Ca2+]i in isolated rat mesenteric artery vascular smooth muscle cells. Pflugers Arch. 1992 Jan;420(1):115–117. doi: 10.1007/BF00378652. [DOI] [PubMed] [Google Scholar]
  3. Baró I., O'Neill S. C., Eisner D. A. Changes of intracellular [Ca2+] during refilling of sarcoplasmic reticulum in rat ventricular and vascular smooth muscle. J Physiol. 1993 Jun;465:21–41. doi: 10.1113/jphysiol.1993.sp019664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ganitkevich VYa, Isenberg G. Ca2+ entry through Na(+)-Ca2+ exchange can trigger Ca2+ release from Ca2+ stores in Na(+)-loaded guinea-pig coronary myocytes. J Physiol. 1993 Aug;468:225–243. doi: 10.1113/jphysiol.1993.sp019768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ganitkevich VYa, Isenberg G. Caffeine-induced release and reuptake of Ca2+ by Ca2+ stores in myocytes from guinea-pig urinary bladder. J Physiol. 1992 Dec;458:99–117. doi: 10.1113/jphysiol.1992.sp019408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grynkiewicz G., Poenie M., Tsien R. Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem. 1985 Mar 25;260(6):3440–3450. [PubMed] [Google Scholar]
  7. Highsmith S., Bloebaum P., Snowdowne K. W. Sarcoplasmic reticulum interacts with the Ca(2+) indicator precursor fura-2-am. Biochem Biophys Res Commun. 1986 Aug 14;138(3):1153–1162. doi: 10.1016/s0006-291x(86)80403-x. [DOI] [PubMed] [Google Scholar]
  8. Itoh T., Kajikuri J., Kuriyama H. Characteristic features of noradrenaline-induced Ca2+ mobilization and tension in arterial smooth muscle of the rabbit. J Physiol. 1992 Nov;457:297–314. doi: 10.1113/jphysiol.1992.sp019379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Missiaen L., De Smedt H., Droogmans G., Himpens B., Casteels R. Calcium ion homeostasis in smooth muscle. Pharmacol Ther. 1992 Nov;56(2):191–231. doi: 10.1016/0163-7258(92)90017-t. [DOI] [PubMed] [Google Scholar]
  10. Morgan J. P., Morgan K. G. Stimulus-specific patterns of intracellular calcium levels in smooth muscle of ferret portal vein. J Physiol. 1984 Jun;351:155–167. doi: 10.1113/jphysiol.1984.sp015239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mulvany M. J., Aalkjaer C., Petersen T. T. Intracellular sodium, membrane potential, and contractility of rat mesenteric small arteries. Circ Res. 1984 Jun;54(6):740–749. doi: 10.1161/01.res.54.6.740. [DOI] [PubMed] [Google Scholar]
  12. Nelson M. T., Standen N. B., Brayden J. E., Worley J. F., 3rd Noradrenaline contracts arteries by activating voltage-dependent calcium channels. Nature. 1988 Nov 24;336(6197):382–385. doi: 10.1038/336382a0. [DOI] [PubMed] [Google Scholar]
  13. Noguera M. A., D'Ocon M. P. Different and common intracellular calcium-stores mobilized by noradrenaline and caffeine in vascular smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 1992 Mar;345(3):333–341. doi: 10.1007/BF00168695. [DOI] [PubMed] [Google Scholar]
  14. O'Neill S. C., Donoso P., Eisner D. A. The role of [Ca2+]i and [Ca2+] sensitization in the caffeine contracture of rat myocytes: measurement of [Ca2+]i and [caffeine]i. J Physiol. 1990 Jun;425:55–70. doi: 10.1113/jphysiol.1990.sp018092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pacaud P., Loirand G., Bolton T. B., Mironneau C., Mironneau J. Intracellular cations modulate noradrenaline-stimulated calcium entry into smooth muscle cells of rat portal vein. J Physiol. 1992 Oct;456:541–556. doi: 10.1113/jphysiol.1992.sp019352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sato K., Ozaki H., Karaki H. Multiple effects of caffeine on contraction and cytosolic free Ca2+ levels in vascular smooth muscle of rat aorta. Naunyn Schmiedebergs Arch Pharmacol. 1988 Oct;338(4):443–448. doi: 10.1007/BF00172125. [DOI] [PubMed] [Google Scholar]
  17. Suematsu E., Hirata M., Kuriyama H. Effects of cAMP- and cGMP-dependent protein kinases, and calmodulin on Ca2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle. Biochim Biophys Acta. 1984 Jun 13;773(1):83–90. doi: 10.1016/0005-2736(84)90552-2. [DOI] [PubMed] [Google Scholar]
  18. Yamamoto H., van Breemen C. Ca2+ compartments in saponin-skinned cultured vascular smooth muscle cells. J Gen Physiol. 1986 Mar;87(3):369–389. doi: 10.1085/jgp.87.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]

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