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. 2024 Nov 20;13:RP94347. doi: 10.7554/eLife.94347

Figure 6. IGF2BP2 regulates the replication of Zika virus (ZIKV) viral RNA (vRNA).

Figure 6.

(A) Schematic representation of reporter ZIKV H/PF/2013 sub-genomic replicons (sgR2A) and replication-deficient genomes because of mutations in NS5 RNA-dependent RNA polymerase (RdRp) sequence (sgR2A GAA). (B–C) Huh7.5 were transduced with short-hairpin RNA (shRNA)-expressing lentiviruses and subjected to electroporation with in vitro-transcribed sgR2A or sgR2A GAA RNAs 2 days later. In-cell bioluminescence was measured (B) 48 or (C) 4 hr post-electroporation and normalized to the non-target shRNA (shNT) control condition. In (C), the luciferase activity was normalized to the transfection efficiency, i.e., the Renilla luciferase (Rluc) activity at 4 hr post-electroporation. Means ± SEM are shown based on four independent experiments. ***: p<0.001; NS: not significant (unpaired t-test).

Figure 6—source data 1. Data points to generate all bar graphs of Figure 6.