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. 2024 Nov 20;13:RP94347. doi: 10.7554/eLife.94347

Author response image 1. DENV NS5 is not specifically co-immunoprecipitated with IGF2BP2-HA in contrast to ZIKV NS5.

Author response image 1.

Huh7.5 cells stably expressing IGF2BP2-HA (+) and control cells (-) were infected with ZIKV H/PF/2013 at a MOI of 10 or left uninfected. Two days later, cell extracts were prepared and subjected to RNase A treatment (+) or not (-) before anti-HA immunoprecipitations. The resulting complexes were analyzed by western blotting for their abundance in the indicated proteins.