Abstract
The photosynthetic cell membrane is impermeable to the oxidized redox dyes Methyl Viologen and Benzyl Viologen, whereas the reduced forms easily penetrate into the cells. By exploiting this permeability difference, the orientation of the membrane-bound hydrogenase has been determined.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adams M. W., Mortenson L. E., Chen J. S. Hydrogenase. Biochim Biophys Acta. 1980 Dec;594(2-3):105–176. doi: 10.1016/0304-4173(80)90007-5. [DOI] [PubMed] [Google Scholar]
- Gogotov I. N., Zorin N. A., Serebriakova L. T., Kondratieva E. N. The properties of hydrogenase from Thiocapsa roseopersicina. Biochim Biophys Acta. 1978 Apr 12;523(2):335–343. doi: 10.1016/0005-2744(78)90036-0. [DOI] [PubMed] [Google Scholar]
- Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
- Jones R. W., Garland P. B. Sites and specificity of the reaction of bipyridylium compounds with anaerobic respiratory enzymes of Escherichia coli. Effects of permeability barriers imposed by the cytoplasmic membrane. Biochem J. 1977 Apr 15;164(1):199–211. doi: 10.1042/bj1640199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. W., Gray T. A., Garland P. B. A study of the permeability of the cytoplasmic membrane of Escherichia coli to reduced and oxidized benzyl viologen and methyl viologen cations: complications in the use of viologens as redox mediators for membrane-bound enzymes. Biochem Soc Trans. 1976;4(4):671–673. doi: 10.1042/bst0040671. [DOI] [PubMed] [Google Scholar]
- Jones R. W. The role of the membrane-bound hydrogenase in the energy-conserving oxidation of molecular hydrogen by Escherichia coli. Biochem J. 1980 May 15;188(2):345–350. doi: 10.1042/bj1880345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondrat'eva E. N., Gogotov I. N., Gruzinskii I. V. Vliianie azotsoderzhashchikh soedinenii na fotovydelenie purpurnymi bakteriiami vodoroda i azotfiksatsiiu. Mikrobiologiia. 1979 May-Jun;48(3):389–395. [PubMed] [Google Scholar]
- Sim E., Vignais P. M. Hydrogenase activity in Paracoccus denitrificans. Partial purification and interaction with the electron transport chain. Biochimie. 1978;60(3):307–314. doi: 10.1016/s0300-9084(78)80827-x. [DOI] [PubMed] [Google Scholar]
- Zorin N. A., Gogotov I. N. Ochistka i svoystva tsitokhroma c552 iz purpurnoi serobakterii Thiocapsa roseopersicina. Biokhimiia. 1980 Aug;45(8):1497–1502. [PubMed] [Google Scholar]